Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings

By Yanfeng Zhang, PhD and Stephanie Cragg, PhD
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Output Details

Description
The steps detailed in this protocol are to label choline acetyltransferase (ChAT) or tyrosine hydroxylase (TH), which are known markers for cholinergic interneurons (Chl) and dopaminergic neurons and striatal axons (DA), respectively. We use an immunofluorescent approach in 300-µm thick slices of mouse brain tissue after performing ex vivo voltammetry recordings.
Identifier (DOI)
10.17504/protocols.io.n2bvj3jw5lk5/v1
Tags
  • Acetylcholine
  • Brain
  • Dopamine
  • Ex Vivo
  • Microscopy - optical
  • Mouse
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

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