Voltage sensor imaging in a population of dopaminergic axons in the mouse striatum

By Lucille Duquenoy, MSc, Bethan O’Connor, BSc and Stephanie Cragg, PhD
View Published Protocol

Output Details

Description
This protocol describes how to perform voltage sensor imaging from a population of striatal dopaminergic axons using high frame rates (600 Hz minimum) on coronal mouse brain slices. In particular, this protocol has been optimised for the use of ASAP5f voltage sensor (AAV9-EF1α-DIO-ASAP5-WPRE) from the Lin lab and changes in fluorescence are resulting from electrical stimulation.  
Identifier (DOI)
10.17504/protocols.io.rm7vzxp6rgx1/v1
Tags
  • Dopaminergic neurons
  • Ex Vivo
  • Genetically encoded biosensors
  • Mouse
  • Other
  • Striatum
  • Voltage Imaging
Team
Team Cragg
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

Related Research

Bulk RNAseq of dopaminergic neurons in vitro cultures

Bulk RNAseq data of hES and hiPSCs differentiated into dopaminergic neurons from ventral midbrain fate were generated using Illumina TruSeq Stranded mRNA kit. Detailed protocols for differentiation can be found at protocols.io.

View Dataset

Anxa1-P2A-Cre Mouse line

Anxa1-Cre knock-in mice express cre recombinase from the mouse Anxa1 promoter in Anxa1-expressing cells and subtypes of dopaminergic neurons. This strain is useful in studies of motor neuron deficits in Parkinson's disease.

View Lab Material

A molecular atlas of cell-type specific signatures in the parkinsonian striatum

Progressive dopamine loss in PD affects striatum cells differently. Transcriptomic analysis in mouse and human models reveals changes in neuronal and glial populations, highlighting resilient and vulnerable cell types for potential new treatments.

View Article
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.