A method for RNA extraction, cDNA synthesis and and quantitative PCR from NIH-3T3 fibroblasts

By Sreeja Nair, PhD and Suzanne Pfeffer, PhD
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Output Details

Description
Here we describe a protocol for RNA extraction, cDNA synthesis and quantitative PCR from NIH-3T3 fibroblasts. This method has also been used with mouse embryonic fibroblasts.
Identifier (DOI)
10.17504/protocols.io.rm7vzjo84lx1/v1
Tags
  • cDNA synthesis
  • qPCR
  • RNA
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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