2D and 3D Neuron/Microglia co-culture systems
Output Details
Description
To visualize iPSC-derived hMG within 2D and 3D co-culture systems, iPSCs are transduced with a lentiviral vector (LV) encoding Green Fluorescent Protein (GFP) under the control of the ubiquitous Phosphoglycerate Kinase (PGK) promoter. After transduction, cells are detached and GFP fluorescence is assessed 72 hours later. Neuron–microglia interactions are examined in a 2D co-culture model using iPSC-derived vmDAn, and confocal microscopy is used to capture images.
Identifier (DOI)
10.17504/protocols.io.3byl4zkzjvo5/v1
