Benzoyl-Hexose derivatization
By onHexose derivatization boosts hexose sugar detection in samples for LC-MS analysis.
ROS Measurement Using CellROX
By onProtocol uses CellROX fluorescent probe to measure cellular oxidative stress via flow cytometry.
Assessment of Mitochondrial OCR For SH-SY5Y Cell using Agilent Seahorse XFe Cell Mito Stress Test
By onProtocol assesses mitochondrial function in SH-SY5Y cells using Agilent Seahorse XFe Cell Mito Stress test measuring OCR. It also examines impact of iron supplementation and ROS inhibition on mitochondrial function.
Mouse Cortical Neuron isolation
By onProtocol for isolating and preparing cortical neurons from E16-17 mouse embryos is outlined in detail.
Flow Cytometry Protocol for LAMP1 Staining V.2
By onProtocol details staining of LAMP1 in human cells using flow cytometry.
PEI transfection protocol for imaging of transient expression of target protein
By onTransfect U2OS cells with PEI for transient expression of GFP-fused protein.
BioID: Identifying Protein-Protein Interactions in Living Cells
By onBioID method uses BirA* to biotinylate proteins near a target protein in cells. This allows isolation of biotinylated proteins for identification using mass spectrometry.
Protocol to generate stable cell line using HEK293 Flp-ln TREx cells
By onHEK293 FlpIn TREx cells were transfected to establish stable cell lines.
Sample preparation for proteomic analysis of LysoIP
By onSample preparation in proteomics is vital for accurate data acquisition and analysis using techniques like LC-MS. Methods range from in-gel digestion to in-solution prep, all following similar principles and steps.
Cloning with restriction enzymes protocol
By onLearn how to efficiently insert your gene fragment into a vector using restriction enzymes for quick cloning.
Convergent molecular pathways to inherited Parkinson’s disease
By onCommon genetic factors for Parkinson's are pathogenic *LRRK2* and *GBA1* variants. LRRK2 affects Rab GTPases, lysosome function, and cilia formation. Inhibiting LRRK2 in mutant mice shows promise in restoring cilia and neuroprotection.
RNA Isolation Using QIAzol-RNeasy Mini Kit and cDNA Synthesis
By onProtocol for RNA isolation using QIAzol and RNeasy Mini columns, followed by cDNA synthesis with High-Capacity cDNA Reverse Transcription Kit. Covers cell harvesting, lysis, purification, and RNA quality for gene-expression studies.
Generation of SLC45A1 Knockout iPSC Line (WTC11-Ngn2 Line)
By onProtocol outlines knockout of SLC45A1 gene in iPSCs using CRISPR-spCas9 system targeting CDS. Can be adapted for other genes.
Measurement of Oxygen Consumption Rate (OCR) in iPSC-Derived Neurons and iPSCs Using Seahorse XFe96 Analyzer
By onProtocol measures OCR in iPSC-derived neurons & undifferentiated iPSCs using Seahorse XFe96 Analyzer.
Protein analyses and immunoblotting
By onProtocol for Western blotting includes cell lysis, protein quantification, SDS-PAGE, protein transfer, antibody incubation, and detection. Enables detection and quantification of target proteins.
Generation of Knockout Cell Lines Using CRISPR–Cas9 Technology
By onProtocol for generating gene knockout cell lines using CRISPR–Cas9 involves sgRNA design, cloning into pX458 vector, transfection, sorting, colony expansion, and validation through Sanger sequencing and ICE analysis.
Virus Production and Transduction Protocol for Retroviral plasmids
By onProtocol for retroviral particle generation using HEK293T cells involves virus production, harvesting, and spin infection for stable gene expression in target cells.
Protocol for Mouse Brain and SH-SY5Y Cell Sample Preparation for Electron Microscopy
By onProtocol for preparing mouse cortical tissue and SH-SY5Y cells for EM is outlined.
Protocol for Lysosomal Hexose Enrichment in SH-SY5Y Cells
By onProtocol enriches hexoses in lysosomes of SH-SY5Y cells by serum starvation, sucrose loading, and invertase hydrolysis.
Data Acquisition and Analysis of Derivatized Hexose/Glucose by LC-QQQ-MS
By onProtocol for analyzing derivatized hexose/glucose with LC-QQQ-MS using a C18 column and Ultivo mass spectrometer. Includes chromatographic conditions, MS settings, and data analysis for high-throughput quantification.
