Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Biochemical analysis of PINK1 activation and ubiquitination signaling upon GTPP treatment
Ubiquitinated proteins were enriched using Halo-m-DSK ubiquitin capture, and GFP-PINK1 complexes were isolated by IP, followed by SDS–PAGE and immunoblotting to assess PINK1 activation and downstream ubiquitination signaling upon GTPP treatment
Tabular data from Mitochondrial Proteostasis Cryo-ET Analysis
Tabular output data from the Mitochondrial Proteostasis Cryo-ET Analysis as demonstrated in Ehses et al., 2026.
Molecular dynamics flexible fitting output of mHsp60 models into in situ density maps
MDFF simulations were performed to evaluate how well atomic models of mHsp60 single rings in different nucleotide states fit the in situ subtomogram averaging density maps by RMSD-based assessment of model–map agreement.
Fluorescence microscopy analysis of PINK1 localization upon GTPP treatment
PINK1-GFP Knock-in cells were stained with the mitochondrial membrane potential–dependent dye MitoSpy Red CMXRos and nuclear DNA dye Hoechst 33342 following treatment with 10µM G-TPP over multiple time points.
ADP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ADP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 half-football complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 half-football complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 bullet complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 bullet complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 football complex (D7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 football complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
SPA dataset of in vitro human mHsp60 assemblies
Dataset used for Single-particle cryo-EM analysis of recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP. Purified proteins were incubated under defined conditions and vitrified at multiple time points.
mtHsp60:mtHsp10 football complex, cryo-ET STA from HeLa
Subtomogram average of the mtHsp60:mtHsp10 football complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
In situ cryo-ET dataset of mitochondria in HeLa cells
Dataset of tilt-series from in situ cry-electron tomography. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
mtHsp60 single- ring complex, cryo-ET STA from HeLa
Subtomogram average of the mtHsp60 single- ring complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
mtHsp60:mtHsp10 half-football complex, cryo-ET STA from HeLa
Subtomogram average of the mtHsp60:mtHsp10 half-football complex, generated from in situ cry-electron tomography data. Images were acquired on FIB-milled HeLa cells that were either treated with 10µM GTPP for 4h or left untreated.
