Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Mass spectrometry proteomics dataset
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD053892
LRRK2 kinase inhibition restores haloperidol effects on rpS6 signaling in iSPNs
IF images in LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons
LRRK2 regulates haloperidol mediated upregulation of immediate early genes in iSPNs
To define the impact of LRRK2 kinase activity on the expression pattern of Nr4a1 mRNA in the SPNs, we used multicolored single-molecule fluorescence in situ hybridization
LRRK2 alters the composition and organization of active zone release sites in dopaminergic striatal synaptosomes
Confocal images and analysis overview
TH fibers and dopamine vesicle clustering alterations
Th fibers and dopamine vesicle clustering alterations in Lrrk2WT and Lrrk2G2019S mice
LRRK2 is expressed in dopamine neurons and is enriched in the vulnerable subpopulation
Confocal images from Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
LRRK2 disrupts the number and composition of active zone release sites in vulnerable dopamine axons
Striatal brain sections were stained with antibodies against bassoon to analyze the number and organization of active zone sites in Aldh1a1+ (EYFP+) or Aldh1a1- (mCherry+) axons from the same mouse
Proteome of R1441C and G2019S LRRK2 knock in mice after striatal motor learning task
To identify the signaling pathways that underlie motor learning deficits, the authors performed six-plex tandem mass tag (TMT) quantitative mass spectrometry (MS) to compare the protein expression in mice following the five days of the rotarod test.
Tabular data of striatal motor learning experiments in LRRK2 KI mice
The tabular data of the behavioral experiments described in the manuscript titled “R1441C and G2019S LRRK2 knockin mice have distinct striatal molecular, physiological, and behavioral alterations.”
Tabular data of fast-scanning voltammetry and physiology experiments in LRRK2 KI mice
The tabular data of fast-scanning cyclic voltammetry and physiology experiments from manuscript: “R1441C and G2019S LRRK2 knockin mice have distinct striatal molecular, physiological, and behavioral alterations.” by Xenias et al.
Parkinson’s Disease MAGMA Analysis Ranked Genes
GWAS summary statistics for PD risk were obtained and processed using the MAGMA tool on FUMA’s online platform to generate a scored list (z-scores) of significant genes.
MERFISH Dataset of Mouse Dopamine Neurons
MERFISH runs of mouse Midbrain spatial transcriptomics
snRNA data from Gaertner & Oram et al., 2024
DAT-Cre mice with a nuclear GFP reporter and either WT or heterozygous G2019S mutant alleles for the gene Lrrk2. Samples contain tissue from midbrains after isolation of DAT+ nuclei via microfluidic sorting for GFP.
Images of mouse VTA and SNc sections labeled for neurotransmitter phenotype markers via RNAscope
48 coronal sections from 3 male and 3 female C57Bl/6J mice were labeled for mRNAs encoding the canonical vesicular transporters for dopamine (VMAT2), GABA (VGAT), and glutamate (VGLUT2). Imaged via confocal microscopy.
Primary Data for Article “Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input” (DOI: 10.1016/j.celrep.2024.114483)
Primary data for the published manuscript "Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input" (Ryan et al. Cell Reports 43:8, 114483, 2024.)
