Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
-
Output Type
-
Program
-
CRN Team Name
-
Theme
KOLF2.1 J (ATP13A2WT)
KOLF2.1 J (ATP13A2WT)
HeLa PINK1-GFP knock-in cell line
A HeLa cell line expressing PINK1 fused to GFP from the endogenous locus was generated using CRISPR–Cas9 nickase–mediated genome editing with homology-directed repair. GFP-positive clones were isolated by fluorescence-activated cell sorting.
pGST2-NDP52
Plasmid for the purification of recombinant GST-tagged NDP52 protein.
pCAG-MBP-ATG9-V836A
Plasmid for expression of human ATG9-V836A mutant in mammalian cells
pCMV-DEAD XPACK-DNAJC5 (L115R)
Mammalian expression of DEAD XPACK-DNAJC5 (L115R)
pHAGE-eGFP-TAX1BP1 CC1Δ
Plasmid
2BT-His-TEV-cs-ATG3_Y210A
DISCONTINUED - Plasmid for bacterial Expression of human ATG3 Y210A.
pHAGE-APEX2-FLAG-LGALS8
Plasmid
Tet-off TauRD (P301L/V337M)
Plasmid: Tet-off TauRD (P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
pHTN-Halo-TEV-ATG3_H266Q
Plasmid for Mammalian Expression of human ATG3 H266Q.
pHTN-Halo-TEV-ATG3_C264S
Plasmid for Mammalian Expression of human ATG3 C264S.
pLenti-CMV-DNAJC5 (L115R)
Mammalian expression of DNAJC5 (L115R)
