Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
HEK293 TMEM115-3xHA GolgiTAG (CVCL_C8A6)
Cell line generated from HEK293 cells (Cat# CRL-1573, RRID:CVCL_0045) virally transfected with a construct expressing TMEM115-3xHA (GolgiTAG)
cDNA clone for bacterial expression of human PPM1H D288A
Plasmid for bacterial expression of human PPM1H (D288A); contains TEV protease site for removal of 6His tag.
A549 PPM1H-BromoTag (CVCL_C8YV)
Cell Line: PPM1H-BromoTag CRISPR/CAS9 A549 knock-in cell line generated from parental A549 cells (ATCC A549 CCl-185) by introducing a BromoTag linked to the C-terminus of PPM1H using CRISPR/Cas9 gene-editing technology.
cDNA clones for expression of GolgiTag (TMEM115) in mammalian cells
Plasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.
cDNA clones for expression of LysoTag (TMEM192) in mammalian cells
Plasmids for expression of TMEM192 (LysoTag) in human and mouse cells.
cDNA clone for bacterial expression of human PPM1H H153D
Plasmid for bacterial expression of human PPM1H (H153D); contains TEV protease site for removal of 6His tag.
cDNA clones for expression of MitoTag (OMP25) in mammalian cells
Plasmids for expression of OMP25 (MitoTag) in human and mouse cells.
A549 PIP4P1 (TMEM55B) KO (CVCL_D3VW)
A549 PIP4P1 (TMEM55B) CRISPR/CAS9 A549 knock-out cell line generated from parental A549 cells (ATCC A549 CCl-185) using CRISPR/Cas9 gene-editing technology. The cell line was verified by immunoblotting analysis and DNA-sequencing analysis. Used in…
HEK293 TMEM192-3xHA LysoTAG (CVCL_D6SR)
LysoTag HEK293 cells for lysosome profiling.
PPM1M knockout Mouse Embryonic Fibroblasts (mouse)
PPM1M knockout mouse embryonic fibroblasts compared to wild-type littermate controls.
PINK1 WT/LRRK2 WT, PINK1 WT/LRRK2 RC, PINK1 KO/LRRK2 WT, PINK1 KO/LRRK2 RC
Mice with LRRK2 R1441C and PINK1 KO mutations were crossed to create double mutant lines: LRRK2 WT/PINK1 WT, LRRK2 RC/PINK1 WT, LRRK2 WT/PINK1 KO, and LRRK2 RC/PINK1 KO.
LRRK2 R1441C/PINK1 Double mutant mouse embryonic fibroblasts
LRRK2 R1441C/PINK1 double mutant mouse embryonic fibroblasts were studied alongside homozygous mutant and littermatched wild-type cells.
pCMV5D HA-PPM1H_M flap
Creating a hybrid protein by combining the HA-PPM1H protein with the flap domain of PPM1M.
His-SUMO-Rab10 Q68L
Sumo-tagged Rab10 with the Q68L mutation
pCMV5D HA-PPM1M_H flap
A hybrid protein combining HA-PPM1M with the flap domain of PPM1H.
