Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
HeLa PINK1-GFP knock-in cell line
A HeLa cell line expressing PINK1 fused to GFP from the endogenous locus was generated using CRISPR–Cas9 nickase–mediated genome editing with homology-directed repair. GFP-positive clones were isolated by fluorescence-activated cell sorting.
pHAGE-eGFP-TAX1BP1 CC1Δ
Plasmid
pHAGE-APEX2-FLAG-LGALS8
Plasmid
Tet-off TauRD (P301L/V337M)
Plasmid: Tet-off TauRD (P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
H9 ES AAVS1-NGN2 FAM134C/A-/-
ES cells modified using CRISPR/Cas9 to lack FAM134C & A ER-phagy receptor. NEUROG2 construct introduced in AAVS1 locus. Derived from human embryonic stem cells at blastocyst stage.
H9 ES AAVS1-NGN2 FLAG-EEA1
ES cells were modified to create iNeurons expressing EEA1 with a 3xFLAG tag. NEUROG2 was introduced at the AAVS1 locus using CRISPR/Cas9. The cells are derived from human embryonic stem cells at the blastocyst stage.
H9 ES AAVS-NGN2; FBXO7-/-
H9 ES cells modified with AAVS1-NGN2 and CRISPR-deleted FBXO7.
HeLa HFT/TO PARK2 FBXO7−/−
HeLa cells with FBXO7/PARK15 deletion made by CRISPR/Cas9.
pHAGE-APEX2-FLAG-LGALS1
Plasmid
pAC150 GFP-RFP-LGALS3
Plasmid
Tet-off Full Length Tau (0N4R, P301L/V337M)
Plasmid: Tet-off Full Length Tau (0N4R, P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
