Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack
Bright green (mNeon) and red (mRuby) fluorescence proteins are expressed, with the former being cytoplasmic and the latter membrane-targeted. This expression is ideal for tracking long diffuse axons in cortical structures in vivo or for slice…
pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA
It is a viral construct designed to express GCaMP6m pan-neuronally and specifically express a red fluorophore (mRuby) in the nucleus of cortical interneurons. This construct is ideal for dual-imaging experiments to capture neuronal activity of two…
pAAV_Cbh_DIO_hTyrNull_HATag
Strong expression of CbH promoter that is non-silencenable, HA tag for histological verification of expression.
pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE
This links GCaMP8f with a ribosome-tethered nanobody that restricts GCaMP to the soma of neurons. Useful in very dense nuclei with strong neuropil, which can make post-hoc cell segmentation difficult.
pAAV_Ef1a_DIO_hTyrHA_minBack
Cre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. Expression under strong Ef1a promoter.
pAAV_Ef1a_DIO_hTyrNull_HATag
Strong expression of Ef1a promoter, HA tag for histological verification of expression.
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
This construct links GCaMP8s to Synaptophysin and directs the fusion protein to presynaptic sites. It also produces a cytoplasmic red fluorescence protein (mRuby) via the self-splicing peptide 2A.
pAAV_Cbh_DIO_hTyr
Cre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. The CbH promoter is a strong, pan-neuronal, non-silencable promoter.
