Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
MANUAL CELL COUNTING
MANUAL CELL COUNTING
FISH CODETECTION
DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
Solutions used for FISH Codetection
Protocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
T cell purification and activation
This protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen
HyDrop Bead Generation & PCR Barcoding V.2
Protocol for producing dissolvable barcoded hydrogel beads used in HyDrop experiments.
A TIRF microscopy-based assay to monitor LRRK2-dependent endo-lysosome exocytosis
Here we describe a method for monitoring CD63+ endo-lysosome exocytosis in hTERT-RPE cells stably expressing CD63-pHluorin.
Lentivirus preparation using Lenti-X Concentrator
This protocol describes the preparation of Lenti-GCaMP6 viral particles using a 2nd generation lentiviral system.
Lenti-jRGECO1b and lenti-iGluSnFR virus preparation
This protocol describes the preparation of EF1-jRGECO1b and EF1-iGluSnFR lentiviral particles using HEK293T cells.
Immunofluorescence and quantification
This protocols describes the immunofluorescence staining and image quantification published in: 3D bioprinting of human neural tissues with functional connectivity DOI: 10.1016/j.stem.2023.12.009
Whole-cell patch-clamp of PSC-derived cortical glutamatergic and GABAergic neurons
This protocol describes whole-cell-patch-clamp of PSC-derived cortical glutamatergic and GABAergic neurons.
Hybrid selection protocol using 10x Single-Cell RNA-Seq assay library
This protocol is for target enrichment of cDNA libraries generated with 10x Genomics single-cell RNA-seq assays.
Membrane Polarization
The protocol describes membrane polarization in Drosophila brains.
Quantifying Acetylcholinesterase (AChE) activity in striatal mouse brain tissue
This protocol describes the steps to measure acetylcholinesterase (AChE) activity in mouse striatal brain tissue.
Chromium single-nucleus RNA Sequencing using the 10x Genomics Chromium Platform
This protocol describes the isolation, fluorescence-activated sorting, and sequencing of nuclei from mouse striatal tissue for single-nucleus RNA sequencing (snRNA-seq) using the 10x Genomics Chromium Next GEM Single Cell 3′ Gene Expression…
GRAB sensor (GRAB ACh or GRABDA) imaging in ex vivo mouse striatal slices
This protocol describes how to image GRAB Acetylcholine (GRABACh) and GRAB Dopamine (GRABDA) sensor expression in ex vivo mouse striatal slices.
