Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Jess western blot automated system
Brief description of preparation of samples for Jess western blot automated system. Detailed instructions are included with the reagent pack
An ImageJ/FIJI Preprocessing Workflow for Multi-Series Confocal Microscopy Datasets Prior To CellProfiler Analysis
A workflow using ImageJ/FIJI aims to create three-slice MIPs from multi-series .czi files to capture weak signals. It involves extracting datasets, creating projections, and saving images for efficient analysis in CellProfiler.
A CellProfiler Pipeline for Quantification of p-SNCA in Mouse Striatal Cholinergic and Medium Spiny Neurons
Brain sections stained for pSNCA, DARPP-32, ChAT, and DAPI. Z-stacks processed into MIPs for analysis. Outputs include pSNCA intensity and neuronal mask area, normalised for cell type-specific burden using R script.
GRIN lens implant (1 mm) Protocol
This is the standard 1 mm GRIN lens implant protocol for Rodrigues-Vaz and Athalye et al, 2025.
Perfusion and Histology Rodrigues-Vaz and Athalye et al, 2025
This is the standard protocol for perfusion and histology of brain slices for Rodrigues-Vaz and Athalye et al, 2025.
EMG recordings-Rodrigues-Vaz and Athalye et al, 2025
This is the standard protocol for recordings of bulk EMG used for Rodrigues-Vaz and Athalye et al, 2025.
Isometric forelimb task behavioral training
This task is a self-paced task in which mice are reinforced for using their forelimb to apply force to an immobile joystick exceeding a threshold of duration and force.
Imaging using 2-photon microscope through GRIN lens
This is the standard protocol for 2-photon imaging with Bruker microscope through GRIN lens used for Rodrigues-Vaz and Athalye et al, 2025.
Holographic calibration
This is the standard protocol for 2-photon holographic calibration with In vivo Ultima Bruker microscope through GRIN lens used for Rodrigues-Vaz and Athalye et al, 2025.
Immunoprecipitation of NAP1-GFP
This protocol describes the procedure to perform immunoprecipitation of NAP1-GFP from HAP1 cells.
Plasmid Construction and Gibson cloning
This protocol describes plasmid construction using Gibson cloning.
Histology Albarran et al 2025
Histology protocol for Albarran et al 2025 that was adapted from Fushiki et al 2024 , to quantify Th+ cells.
Cryo-electron tomography of thinned synapses
Protocol for cryo-ET of thinned synapses in rat neuron cultures is detailed. Cryo-FIB milling creates thin samples for high-resolution imaging. Synapse targeting with and without cryo-FLM correlation is explained.
Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography
Sandwich hippocampal neuron cultures have low cell density and few glial cells, making them ideal for targeting neurons for cryo-electron tomography.
Single particle analysis of α-Synuclein fibrils
Aggregates of α-Synuclein are linked to Parkinson’s, multiple system atrophy, and Lewy body dementia. This protocol outlines near-atomic resolution structures of these amyloid fibrils using cryo-EM single particle analysis.
