Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Protocol to study synapse density or volume— SynDOVE—in brain using confocal microscopy and Imaris three-dimensional surface rendering software
Guide for analyzing for analysis of synaptic loci density and pre- and postsynaptic volumes (SynDOVE). from confocal images of pre- and postsynaptic loci in fixed brain sections with Imaris software.
CXCL1 supplementation on iPSC-Derived Midbrain Neurons
The protocol studies the impact of different levels of recombinant human CXCL1 on midbrain neurons from iPSCs using conditioned media from ATP13A2WT astrocytes.
Bulk RNA-seq Protocol for Astrocytes
Protocol for RNA extraction from astrocytes for bulk RNA sequencing to analyze gene expression profiles.
Bisulfite-seq for Astrocytes
Bisulfite-seq for Astrocytes
ATAC-seq Protocol for Astrocytes
ATAC-seq Protocol for Astrocytes
SAM ELISA
Protocol for preparing cell lysates and conducting ELISA to quantify proteins is outlined.
Fluorescently labeled polyamine uptake (via confocal microscopy)
Method visualizes polyamine uptake in cells with fluorescently labeled polyamines & confocal microscopy.
CXCL1 ELISA
Protocol for ELISA includes sample prep & assay to quantify antigens in biological samples.
Fluorescently labeled polyamine uptake (via flow cytometry)
Supplemental video in ASAP Protocol Particulars series provides extra context and tips from protocol authors for Aligning Science Across Parkinson's project.
Differentiation of iPSCs into NGN2 Neurons Protocol
Protocol uses doxycycline to induce neurogenin 2 in iPSCs for neuron differentiation, aiding research on neuronal development and function.
Human Induced Pluripotent Stem Cell (hiPSC) Culture and Passaging Protocol
Protocol for hiPSC maintenance and passage using Geltrex-coated plates and StemFlex medium for optimal growth and reduced differentiation.
Differentiation of Human Induced Pluripotent Stem Cells into Midbrain Neurons
Protocol uses specific media and growth factors to convert hiPSCs into midbrain neurons with high purity and reproducibility for transplantation and disease modeling.
Protocol for Dissociation and Culture of Midbrain Astrocytes and Neurons
Protocol for isolating and culturing midbrain astrocytes and neurons in specific ratios to preserve viability and differentiation potential for future research purposes.
Astrocyte-Conditioned Media (ACM) cultures
Astrocyte-conditioned media (ACM) is created for research on D25 midbrain organoids and 2D neuronal cultures.
Protocol for Live-Cell Imaging of Astrocytes Exposed to HiLyte™ 488-labeled Human Recombinant α-Synucleins
Protocol studies impact of HiLyte™ 488-labeled human recombinant α-synuclein on astrocytes through live-cell imaging at different time points post-exposure.
