Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
A 4-5X expansion microscopy protocol for high‑resolution imaging of olfactory sensory neuron cilia in mouse olfactory epithelium
Protocol for expanding mouse olfactory epithelium for improved imaging: OE dissected on a cold platform, embedded in hydrogel, and expanded 4-5 times with ExM. Enables detailed visualization of ciliary structures without super-resolution microscopy.
Binding of Rab29 to the LRRK2 Armadillo domain by Microscale Thermophoresis
Microscale thermophoresis is a powerful tool to measure the affinities of interactions between proteins. We present here our method for determining the binding of Rab29 GTPase to the LRRK2 N-terminal Armadillo domain.
Staining of cells with GolgiTracker for Golgi flow cytometry analysis
Method that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis.
Untargeted metabolomics analysis for Golgi immunopurification (Golgi-IP)
This protocol provides details for analyzing GolgiIP metabolomics samples using liquid chromatography mass spectrometry (LC-MS) for polar metabolite profiling.
Golgi immunopurification (Golgi-IP) for subcellular metabolite profiling
This protocol provides details for preparing Golgi-IP metabolomics samples.
Co-immunoprecipitation protocol to study LRRK2 binding to Rab12 in a cell-based assay
The immunoprecipitation of FLAG-tagged LRRK2 from whole cell lysates to assess its interaction with Rab12.
Immunofluorescence imaging of cells expressing GolgiTAG (TMEM115-3HA) or HA-tagged non-Golgi annotated proteins
A method that can be used to verify the correct localisation of HA-tagged Golgi proteins by assessing their colocalization with known Golgi markers. olocalization with ACBD3.
Untargeted lipidomics analysis for Golgi immunopurification (Golgi-IP)
This protocol provides details for analyzing GolgiIP lipidomics samples using liquid chromatography mass spectrometry (LC-MS) for nonpolar lipid profiling.
Protocol for Data Independent Acquisition – Mass spectrometry analysis – a DIA-based Organelle Proteomics
Here the authors provide a detailed protocol for the Data Independent Acquisition (DIA)-based mass spectrometry (MS) data acquisition method for proteomic profiling of the Golgi.
Introducing GolgiTag to Cells and Immunoprecipitation of Golgi
Protocol for generating GolgiTag cells and immunoprecipitation of Golgi for subsequent -omics analyses.
Golgi immunopurification (Golgi-IP) for subcellular lipid profiling
This protocol provides details for preparing Golgi-IP lipidomics samples.
Generation of stable cell lines via retroviral or lentiviral transduction
This protocol details how to generate stable cell lines using a retrovirus system
Creating pooled CRISPR-Cas9 knock-outs in NIH-3T3 cells
To validate a genome wide CRISPR screen, the authors select the top hits and create lentiviruses to validate the hits.
Reconstitution of LRRK2 membrane recruitment onto planar lipid bilayers
A method to monitor the recruitment of purified LRRK2 kinase onto planar lipid bilayers containing lipid-anchored Rab10 protein using Total Internal Reflection Fluorescence (TIRF) Microscopy.
Assay for Dual Rab GTPase binding to the LRRK2 Armadillo Domain
The LRRK2 Armadillo domain contains multiple Rab GTPase binding sites. This assay shows that the sites can be occupied simultaneously.
