Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
SNP Genotyping and ApoE Genotyping
Protocol outlines DNA extraction from blood, quality control, SNP, and APOE genotyping. Adapted from PRoBaND SNP Genotyping and ApoE Genotyping Protocol by Malek et al. for Parkinson's Disease study.
CD8 Cell Density In Substantia Nigra And Cerebral Peduncle Image Analysi
QuPath is a bioimage analysis software designed for digital pathology and whole slide image analysis. This protocol describes how to measure CD8 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease: Bioinformatic Prioritisation and Hit Validation
This protocol describes the Bioinformatic Prioritization of PD GWAS candidates for High Content Screening, and Hit Validation by allele-specific expression (ASE) analysis.
Protein interaction network analysis for Mendelian diseases
This protocol describes the steps to use experimentally validated human data to create a protein-protein interaction network (PPIN) based on disease causative genes. Network analysis (combination of topological functional analyses) will lead to the…
Western blotting for LRRK2 signalling in macrophages
This protocol describes the immunoblotting for components of the LRRK2 signalling pathway (LRRK2, LRRK2 pS935 and phospho-Rabs) using Invitrogen NuPage SDS-PAGE reagents and the BioRad Turbo Blot transfer system.
Luminescent Conjugated Oligothiophenes (LCO) Staining
The protocol describes Luminescent Conjugated Oligothiophenes staining on brain sections.
Glucosylceramidase Beta (GBA) Genotyping
This protocol details the steps for GBA genotyping. This protocol has been adapted from the PRoBaND Clinical Consortium (incorporating methods described by Neuman et al., 2009 and Stone et al., 2000) and has been used for all publications for…
Analysis of glycosphingolipids from human plasma
This is an updated protocol with the focus on achieving sensitive and reproducible quantitation of glycosphingolipids from human plasma samples
Immunofluorescence Staining
The protocol describes immunofluorescence staining on brain sections.
Nuclear Isolation of Post-Mortem Brain Tissue for snRNAseq
This protocol details how to isolate nuclei from frozen brain tissue for single nuclear RNA sequencing using 10x Genomics GEM isolation using the Chromium accessory and Single Cell 3ʹ Reagent Kits.
LRRK2 Immunofluorescent staining
Protocol for immunofluorescent staining for LRRK2 in cultured cells using the MJFF2 (c41-2) antibody.
AT8 Tau Pathology Image Analysis
This protocol describes how to analyze AT8 tau pathology in human brain tissue (FFPE sections with IHC).
Automated Immunohistochemistry Staining
The protocols describe the steps for automated immunohistochemistry staining using the Ventana Discovery® XT Immunostainer.
CD3 Cell Density in Substantia Nigra and Cerebral Peduncle Image Analysis
This protocol describes how to measure CD3 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
In Silico analysis links the NSL complex to Parkinson’s disease and the mitochondria – Protein-protein interaction data to functional enrichment analysis
A bioinformatics approach taken to investigate the interactome of the NSL complex, to unpick its relevance to Parkinson’s disease progression.
