Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
-
Output Type
-
Program
-
CRN Team Name
-
Theme
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease: Bioinformatic Prioritisation and Hit Validation
This protocol describes the Bioinformatic Prioritization of PD GWAS candidates for High Content Screening, and Hit Validation by allele-specific expression (ASE) analysis.
SoRA microscopy protocol for imaging oligomers in human brain tissue
This protocol gives a step-by-step guide to imaging oligomers in human brain tissue using a spinning disk confocal microscope.
Live-cell imaging; mitochondrial reactive oxygen species
This protocol presents instructions for assessing mitochondrial souced reactive oxygen species using MitoTracker® Red CM-H2XRos dye.
Single-molecule Immunofluorescence Tissue Staining Protocol for Oligomer Imaging V.3
This protocol details background fluorescence quenching and immunofluorescence staining of human brain tissue for oligomer imaging.
Free-floating Mouse Brain Immunohistochemistry
This protocol enables immunohistochemical staining of murine tissue with superior penetration of the tissue by the reagents due to the free-floating approach.
High-Throughput Multiplex Live-Cell Imaging of iPSC-derived neurons
High-throughput imaging of live iPSC-derived neurons using multiplexed dyes shows dynamic organelle movements in nucleus, mitochondria, and lysosomes.
Single-Molecule Antibody Slides For Fluorescence Microscopy
This protocol describes how to create single-molecule antibody slides for fluorescence microscopy.
Tetraspeck Bead Imaging V.2
Protocol for imaging tetraspeck beads on glass coverslips.
ASO transfection of iPSC-derived cells
A protocol for transfecting iPSC-derived midbrain dopaminergic neurons with antisense oligonucleotides (ASOs).
Aggregation of human recombinant alpha-synuclein
This protocol describes a method of generating aggregation of human recombinant alpha-synuclein.
Single-cell RNA-seq
Harvesting and performing single-cell RNA-seq.
Electrophysiology with iPSC-derived neurons
This protocol describes the method to perform patch-clamp recordings of iPSC-derived neurons.
Bulk tissue RNA-sequencing – hiPSC-derived astrocytes, neurons and co-cultures
Bulk tissue RNA-sequencing of hiPSC-derived astrocytes, neurons and co-cultures using the Illumina TruSeq Stranded mRNA Library Prep kit
MSD V-PLEX Proinflammatory panel
The V-PLEX panel offers analytically validated multiplex assay kits. Developed under rigorous design control, V-PLEX kits provide accurate and reproducible results with consistency from lot to lot.
TEM: Transmission electron microscopy
TEM protocol.
