Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Whole mount dissection and staining of enteric nervous system
This protocol details whole mount dissection and staining of the enteric nervous system.
Regional Mouse Brain Analysis (Modified QUINT)
This is series of protocols that has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow: QuPath visualization/segmentation QuickNII Brain Atlas Registration QMask Hemispheric Separation Visualign…
Embryonic/Postnatal Mouse Neuron Culture Protocol
This protocol details the primary neuron culture procedures from embryonic and postnatal mice.
Hematoxylin and Eosin Stain – FOR PARAFFIN-EMBEDDED TISSUE SECTIONS
Protocol for Hematoxylin and Eosin Stain on paraffin-embedded tissue sections is described.
Human Brain Sequential Extraction (Tau)
Human brain sequential detergent fractionation for PHF tau extraction
Direct ELISA
The protocol explains how to perform a direct ELISA on a specific protein of interest, outlining the setup and running procedures.
α-Synuclein Protein Preparation (Large scale)
Protocol for large-scale preparation of recombinant α-synuclein protein.
Stereotaxic Injections- Mouse
This protocol details mouse stereotaxic injections for any purpose, but in this case is primarily used for the injection of misfolded proteins.
Stereotaxic injections into mouse brain and ex-vivo electrophysiology
Protocol outlines injection of α-Synuclin PFF and monomer into mouse brain, followed by preparation of acute slices from mice for whole-cell patch clamp recordings.
Immunocytochemistry (Henderson Lab)
Protocol for immunocytochemistry on mammalian cells in 24 and 96 well plates.
Three antibody staining protocol for immunolabeling of mouse brain sections
The protocol addresses factors affecting imaging of tissue sections: autofluorescence and consistency in antibody preparation for ideal results.
Synaptic analysis workflow using SEQUIN
Protocol for analyzing synaptic loci in neuroscience experiments using Zeiss Airyscan microscope. Includes identification, quantification, and localization of synaptic loci and nearby labels on neurites of the super-resolution images acquired
Culturing Primary Hippocampal Neurons from Embryonic Rat Brain
Protocol for culturing hippocampal primary neurons from embryonic rats is described.
