Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinsonās disease through collaboration, research-enabling resources, and data sharing. Weāve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
-
Output Type
-
Program
-
CRN Team Name
-
Theme
Fabrication and calibration of multi-fiber arrays to monitor dopamine release in the mouse striatum
A new method allows monitoring dopamine release in mice with high spatial and temporal resolution in multiple locations in the striatum simultaneously using a fluorescent sensor. The protocol involves array fabrication and pre-implant mapping.
Micro-CT scanning for post-implant localization of multi-fiber arrays in mouse striatum
New micro-fiber array allows chronic measurement and optogenetic manipulation at 100+ locations in mice, for studying cell-type and neurotransmitter-specific signals in 3-D volumes. Protocol involves micro-CT scanning and fiber localization.
Confocal microscopy to assess TelC and iGluSnFR expression in fixed mouse brain slices
Protocol outlines tissue processing after glutamate recordings with iGluSnFR sensor & behavior experiments post TelC viral injection in dorsal medial striatum.
Multi-fiber photometry in head-fixed mice performing a dual-cue delay Pavlovian association
Protocol describes dual-cue Pavlovian association & multi-fiber photometry in head-fixed mice. Mice trained to link cues with rewards & undergo extinction phase with reduced rewards.
Stereotaxic injections of viral vectors and chronic optical fiber implants in mouse brains
Protocol details surgical steps for viral injections and optical fiber implantation in mouse striatum to monitor neurotransmitter dynamics during learning and extinction, using genetic sensors and tetanus toxin to suppress acetylcholine release.
Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
This protocol describes the maintenance and expansion of iPSCs in the adherent culture via single-cell passaging.
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
This protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
This protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
This protocol generates human medium spiny neurons (MSNs) from induced human pluripotent stem cells (iPSCs).
Quantification of Extracellular š¶-synuclein Assay
This protocol uses a classical ELISA experimental design with sensitive electrochemical detection to provide measure of š¶-synuclein concentration in the conditioned media of cultured iPSC-derived human cells.
Whole-cell Patch-Clamp Recordings from Striatal Cholinergic Interneurons in ex vivo Mouse Brain Slices
Protocol for patch-clamp recording of mCherry-labelled striatal ChIs from mouse brain slices with added step for labelling astrocytes using SR101.
Visualisation and quantification of dendritic spines in cultured human Medium Spiny Neurons (MSNs)
This protocol describes the visualisation of dendritic spines of human neurons cultured on coverslips in vitro and subsequent quantification using the software Imaris.
Custom open-chamber microfluidic fabrication
This protocol described the fabrication of two- and three-open chambered microfluidics suitable for cell culturing using commercially acquired master mould.
Quantifying Acetylcholinesterase activity in striatal brain tissue with DTNB assay
This protocol describes the steps to measure acetylcholinesterase (AChE) activity in mouse striatal brain tissue, including how to store tissue samples, extract AChE and obtain Michaelis-Menten like plots to measure the rate of AChE activity.
Whole-cell patch-clamping of cultured human neurons
This protocol describes procedures of the whole-cell patch clamping of human neurons derived from induced pluripotent stem cells and cultured in adherent monolayer.
