Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Nuclear cytoplasmic fractionation
This protocol describes nuclear cytoplasmic fractionation.
iPSC differentiation into Macrophages
This protocol describes iPSC differentiation into macrophages.
DQ-BSA quantification
This protocol describes DQ-BSA quantification.
Generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
This protocol is to help with generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
Surface protein biotinylation
This protocol describes surface protein labeling with biotin using EZ-Link Sulfo-NHSLC-Biotin. This chemical reacts with primary amines such as lysine but does not permeate cell membranes because of the charge. Thus, it only biotinylates surface…
RNA isolation and qRT-PCR
This protocol describes the RNA isolation from cultured cells and quantitative RT PCR.
Protein purification
This protocol details how to purify 3xFLAG-SHIP164Δ901-1099
DQ-BSA assay
This protocol describes the DQ BSA assay for measuring the lysosomal proteolytic activity.
Cell culture, transfection, and imaging
This protocol describes general procedures for culturing HeLa cells, transient transfection, and imaging using an Andor Dragonfly spinning disk confocal system.
Subtomogram averaging and classification
This protocol describes the procedure of subtomogram averaging and 3D classification of VPS13C rod-shaped densities inside cryotomograms of mammalian cells. Sub-tomogram averaging package i3 was used for 3D alignment and classification.
Regular maintenance of human pluripotent stem cells
This protocol describes the regular maintenance and passaging human iPSCs.
Ectopic receptor & endosome size and count image analysis and quantification
This protocol is to help with the imaging analysis of ectopic receptor localization and endosome size.
Crystallization, data collection and structure solution of PxP-VAB
Protocol lists the steps to obtain PxP(Mcp1ct)-VABct crystals, X-ray diffraction data acquisition and obtaining the structure solution using molecular replacement with a model generated by AlphaFold2.
siRNA-mediated knockdown of TFE3
This protocol details siRNA-mediated knockdown of TFE3.
Lipid transfer assay
This protocol details how to perform a lipid transfer assay with 3xFLAG-SHIP164Δ901-1099
