Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
AAV viral DNA and whole RNA recovery for AAV pool experiments in rhesus macaque
This protocol outlines procedures to extract viral DNA and whole RNA from rhesus macaque tissue that had been treated with AAV in vivo
Immunohistochemistry of AAV-treated tissues
Outlines procedures to perform immunohistochemistry on tissue that had been treated with AAV.
Tissue handling for mice, marmoset, and rhesus macaques injected with AAV
This protocol outlines all procedures for handling of tissue of animal subjects treated with AAV.
Hippocampal Cultures, lentivirus production, vGLUT1-pHluorin imaging and analysis
Procedures for hippocampal cultures, lentivirus production, vGLUT1-pHluorin imaging and analysis used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
αSyn Immunohistochemistry
Procedure for immunohistochemistry used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Proximity Ligation Assay (PLA)
Procedure for proximity ligation assay used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Immunofluorescence and object-based colocalization analysis
Procedures for immunofluorescence and object-based colocalization analysis used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Preparation and Administration of Systemic 4-AP
Procedure for preparation and systemic administration of 4-AP used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Pull-down Assays
Protocol for protein pull-down assays as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)
GST fusion protein production
Protocol for GST fusion protein production as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)
Immunoprecipitations and Western blots analysis
Protocol for immunoprecipitation and Western blotting as performed in "Synapsin E-domain is essential for α-synuclein function." (https://doi.org/10.7554/eLife.89687.1)
pHluorin assays, analysis, and fluorescence microscopy
Protocol for pHluorin assays, analysis, and fluorescence microscopy as performed in “Synapsin E-domain is essential for α-synuclein function” (https://doi.org/10.7554/eLife.89687.1)
CRISPR knock-in validation
Procedure for CRISPR knock-in validation used in "Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function."
Proteome analysis
Procedure for proteome analysis used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function”
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
Procedure for Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) used in “Serine-129 phosphorylation of α-synuclein is a trigger for physiologic protein-protein interactions and synaptic function”
