Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
FASTQ alignment, gene counts, and differential expression analysis and functional annotation of DEGs
Summary: The process involves aligning FASTQ files, counting genes, analyzing differential expression, and annotating differentially expressed genes for functional insights.
Patient PBMC flow cytometry
Flow cytometry analysis of peripheral blood mononuclear cells (PBMC) from a patient was performed.
Patient PBMC differentiation to macrophages
Differentiation of patient peripheral blood mononuclear cells (PBMCs) into macrophages is a common method used in research to study immune responses and diseases.
pH-Rodo pathogens to monitor phagocytosis in pMacs
pH-Rodo pathogens are used to track phagocytosis in peritoneal macrophages (pMacs).
DQ-BSA and BMV109 microscopy
DQ-BSA and BMV109 microscopy used for studying peritoneal macrophages.
Ea(52-68) uptake assays
Ea(52-68) uptake assays were performed on peritoneal macrophages (pMacs).
Flow cytometry for ex vivo stimulated pMacs
Flow cytometry used to analyze ex vivo stimulated peritoneal macrophages for research purposes.
Harvesting and culturing of peritoneal macrophages and ex-vivo stimulation
The process involves harvesting and culturing peritoneal macrophages followed by ex-vivo stimulation with IFNy for research purposes.
Y-Maze Protocol
The Y maze assesses rodents' spatial working memory by observing their tendency to explore new arms over revisiting old ones. Rodents with intact memory will alternate between arms before revisiting.
STC-1 Passaging and PD Associated Microbe Stimulation Protocol
Protocol for passaging STC-1 cells involves stimulating with microbes to induce proliferation. This process can be used to study cell behavior and responses.
SOP for RT (Reverse Transcription) Promega kit
The SOP for the Promega RT kit includes steps for reverse transcription of RNA to cDNA using provided reagents and protocols for accurate gene expression analysis.
SOP for Immune cells isolation mouse adult brain with CD45 beads
The SOP outlines the procedure for isolating immune cells from adult mouse brain using CD45 beads for subsequent analysis or experiments.
SOP for DSS kinetics studies
The SOP for DSS kinetics studies outlines procedures for conducting and analyzing kinetic experiments using a stopped-flow spectrophotometer.
SOP FOR MESOSCALE DISCOVERY ASSAYS
Standard Operating Procedure (SOP) for conducting mesoscale discovery assays, detailing the step-by-step process for accurate and efficient experimentation.
SOP for Flow cytometry after DSS-induced gut and brain injury
Standard Operating Procedure (SOP) for conducting flow cytometry analysis on samples from mice with DSS-induced gut and brain injuries.
