Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Indirect Proximity Ligation Assay (PLA) – Brightfield
The authors describe the PLA protocol that is routinely used in the laboratory to detect nitrated alpha-synuclein and nitration of mitochondrial enzymes such as SOD2 and the mitochondrial complex 1 subunit NDUFB8.
qPCR of α-synuclein, TNF and NF-κβ
A procedure for quantitative real time reverse transcription PCR of α-synuclein, TNF, and NF-κβ.
STC-1 cell culture
Cell culture of STC-1 mouse enteroendocrine cells.
Immunohistochemistry using paraffin embedded tissue
Immunohistochemistry using paraffin embedded tissue
Generation of induced pluripotent stem cells and gene correction
iPSC generation and gene correction (CRISPR-CAS9) protocol.
Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”
This is a collection of protocols used in a recent preprint by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1
Complex I activity assay
This protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly…
Plasmid-reprogramming of human fibroblasts
This protocol is part of a collection of protocols for the paper, "Glucocerebrosidase, a Parkinson's disease-associated protein, is imported into mitochondria and regulates complex I assembly and function"
Collection of protocols of Team Deleidi used in the publication: “”LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease””
Collection of protocols of Team Deleidi used in the publication: ""LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease""
Validation of Genotyping Method for L444P Mice Ear-Clips.
|| Team Schapira || Authors Revi Shahar Golan, David Chau Abstract Aim: the genotyping is used to identify if mice are heterozygote (hetero) or Wild-Type (WT), and the aim of the work is to validate the digestion method, and PCR program, the PCR…
Immunofluorescence staining
This protocol describes the immunofluorescence staining of cells.
Differentiation NPCs to Dopaminergic/Midbrain Neurons
This protocol details methods for differentiation of NPCs to Dopaminergic/Midbrain Neurons. This protocol is part of a Collection of protocols (dx.doi.org/10.17504/protocols.io.8epv593dng1b/v1) for the paper “Glucocerebrosidase, a Parkinson´s…
Gcase co-immunoprecipitation
The authors developed this protocol to identify protein-protein interactions between the enzyme glucocerebrosidase (GCase) and other proteins in human iPSC-derived Neural Precursor Cells.
Flag co-immunoprecipitation
Protocol used to pull down V5-FLAG-Gcase interacting proteins in HEK cells
Midbrain organoid generation from mfNPC
Optimised midbrain organoid generation from mfNPC.
