Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Nuclei Isolation and Sorting from Frozen Human Temporal Cortex V2
Protocol focuses on isolating and sorting nuclei from frozen human temporal cortex.
Confirming circRNA expression by qPCR
This protocol delineates a qPCR method to confirm the expression of circRNAs extracted from brain samples.
FM1-43 endocytic uptake assay in HIPSC derived neurons
FM1-43 is a lipophilic styryl dye, nontoxic to the cells, virtually nonfluorescent in aqueous media. The dye will become fluorescent once it binds to the plasma membrane. In neurons that are actively releasing neurotransmitters, these dyes become…
Transferrin uptake assay to measure Clathrin-mediated endocytosis in HIPCS derived neurons
This protocol is used to measure Transferrin uptake as a way to investigate Clathrin-mediated endocytosis in HIPCS derived neurons, or other cell types.
Sample Collection and Processing for RNA Analysis
This protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.
