Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Ventral midbrain dopaminergic (mDA) Neuron Differentiation Protocol
Optimized protocol for generating authentic ventral midbrain dopaminergic neurons from human iPSCs enhances cell therapy and modeling, improves consistency across lines, and supports integration with organoid and assembloid systems.
Formation of ventral midbrain Assembloid/Organoids
This pilot establishes brain organoids from iPSC-derived vmDA neurons, optimizing growth conditions and exploring tri-culture integration with microglia to model neurodevelopment and cell-type interactions.
Protocol selection for Saurat et al. CSC 2024 V.2
Protocol collection for the study "Genome-wide CRISPR screen reveals neddylation's role in neuronal aging and AD neurodegeneration" by Saurat et al. in Cell Stem Cell 2024. Contains 20 protocols. Full manuscript: DOI: 10.1016/j.stem.2024.06.001.
Protocol selection for Kim et al. Cell 2024 V.2
Kim et al. in Cell 2024 published "TNF-NF-kB-p53 axis restricts in vivo survival of hPSC-derived dopamine neurons" with 13 protocols. Full text at [DOI: 10.1016/j.cell.2024.05.030](https://doi.org/10.1016/j.cell.2024.05.030).
Immune stimulation of human induced pluripotent stem cells (hiPSC)-derived glia with lipopolysaccharide (LPS)
hiPSCs are used to model human development and diseases. Lipopolysaccharide activates immune cells and triggers cytokine secretion. LPS activates toll-like receptor 4 and NFkB. Protocol how to use LPS to activate iPSC-derived microglia.
Poly-ornithine/laminin substrate for neural cell culture V.2
Protocol to generate a sticky substrate for neurons.
hPSC Passaging and Propagation on laminin521 V.1
Highly efficient passaging and propagation of human PSC.
Seeded amplification assay (SAA) from neuronal cell lysates
Seeded amplification assay (SAA) from neuronal cell lysates
Induced astrocyte differentiation
Induced astrocyte differentiation
Immunofluorescence and microscopy of iPSC-derived cortical neurons
Immunofluorescence and microscopy of iPSC-derived cortical neurons protocol for the preservation of lipid-rich inclusions.
Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
Detection of minute amounts of misfolded α-synuclein aggregates in neuronal cell lysates.
Induced Cortical Neuron Differentiation – Part 3 – Day 7 Dissociation
Protocol for replating neurons after seven days of cortical transdifferentiation and prepare cells for longer-term culture.
Induced Cortical Neuron Differentiation – Part 1 – Overview
Protocol to differentiate human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puro-SNAP PiggyBac construct, with a blasticidin-resistance backbone.
Induced Cortical Neuron Differentiation – Part 2 – Day 0 Dissociation
Protocol to initiate differentiation of human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puroSNAP PiggyBac construct, with a blasticidin-resistance backbone.
Western blot of amplified fibrils after Proteinase K digestion
Protocol for western blot of amplified fibrils of a-synuclein after proteinase K digestion.
