Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Immune stimulation of human induced pluripotent stem cells (hiPSC)-derived glia with lipopolysaccharide (LPS)
hiPSCs are used to model human development and diseases. Lipopolysaccharide activates immune cells and triggers cytokine secretion. LPS activates toll-like receptor 4 and NFkB. Protocol how to use LPS to activate iPSC-derived microglia.
Poly-ornithine/laminin substrate for neural cell culture V.2
Protocol to generate a sticky substrate for neurons.
hPSC Passaging and Propagation on laminin521 V.1
Highly efficient passaging and propagation of human PSC.
Seeded amplification assay (SAA) from neuronal cell lysates
Seeded amplification assay (SAA) from neuronal cell lysates
Induced astrocyte differentiation
Induced astrocyte differentiation
Immunofluorescence and microscopy of iPSC-derived cortical neurons
Immunofluorescence and microscopy of iPSC-derived cortical neurons protocol for the preservation of lipid-rich inclusions.
Seeded amplification assay (SAA) from neuronal cell or postmortem brain lysates
Detection of minute amounts of misfolded α-synuclein aggregates in neuronal cell lysates.
Induced Cortical Neuron Differentiation – Part 3 – Day 7 Dissociation
Protocol for replating neurons after seven days of cortical transdifferentiation and prepare cells for longer-term culture.
Induced Cortical Neuron Differentiation – Part 1 – Overview
Protocol to differentiate human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puro-SNAP PiggyBac construct, with a blasticidin-resistance backbone.
Quantitative PCR for STMN2 expression in pi-N neurons
Quantitative PCR for STMN2 expression in pi-NSNCA-STMN2 neurons.
Induced Cortical Neuron Differentiation – Part 2 – Day 0 Dissociation
Protocol to initiate differentiation of human iPS cells into cortical neurons by doxycycline induction of NGN2 transgene expression. Use iPSCs transfected with an NGN2-puroSNAP PiggyBac construct, with a blasticidin-resistance backbone.
Western blot of amplified fibrils after Proteinase K digestion
Protocol for western blot of amplified fibrils of a-synuclein after proteinase K digestion.
Seahorse XF Cell Mito Stress Test
Seahorse XF Cell Mito Stress Test for assessment of oxygen consumption rate in iPSC-derived neurons as a measure of mitochondrial respiration.
Whole cell protein extraction and Western blotting
This protocol describes cell lysis for whole-cell protein extraction followed by western blotting using LI-COR Odyssey quantitative western blot.
PFF seeding in U2OS cells
This protocol describes the seeding of intracellular inclusions in U2OS cells harboring overexpressing a-synuclein via exogenous addition of short-length preformed a-synuclein fibrils.
