Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Immunohistochemistry (IHC)
Immunohistochemistry for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.
Activation Induced Marker (AIM) Staining Protocol
This protocol details about activation induced marker staining.
Fluorescence in situ hybridization (FISH)
Fluorescence in situ hybridization (FISH) in Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain
Intracellular Cytokine (ICS) Staining Protocol
This protocol details about intracellular cytokine (ICS) staining.
Stereotaxic Injections
Stereotaxic injections for Conserved and cell type-specific transcriptional responses to IFN-γ in the ventral midbrain.
Virus Production and Administration
Protocol for AAV production and administration to mice.
Neural activation of the GI tract
Protocol for neuronal activation of the enteric nervous system.
Tissue preparation, immunohistochemistry, Imaging, and Quantification
Protocol for neuronal imaging
GCaMP6f Fluorescence in Ex Vivo Intestinal Preparation
Protocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.
Metagenome Preparation and Analysis
Protocol for metagenomic analysis from mouse fecal pellets.
Metabolomics Preparation and Analysis
Protocol for metabolomic analysis from mouse fecal and cecal samples.
Proteome Preparation and Analysis
Protocol for proteomic analysis from mouse cecal samples.
3′ mRNA-sequencing
Protocol for 3′ mRNA sequencing of mouse gastrointestinal tract tissues
Whole Gut Transit Time, Fecal Water Content, and Fecal Output
Protocol for mouse gastrointestinal function assays (whole gut transit time, fecal water content, and fecal output)
Colonic Migrating Motor Complexes
Protocol for ex vivo assay of colonic migrating motor complexes (CMMCs) in mouse colons.
