Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Lipidomic analysis in HeLa cells for HexCer levels
A protocol for the lipidomics analysis in HeLa cells for HexCer levels is described.
Lipidomic analysis in HeLa cells
A protocol for the lipidomic analysis in HeLa cells is described.
Lipidomic analysis in WM115 cells
A protocol for the lipidomic analysis in WM115 cells
Midbrain organoid differentiation in spinner flasks
Midbrain differentiation protocol using spinner flasks
Radiolabeled polyamine uptake in cells
This protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.
Fluorescently labeled polyamine uptake (via Flow Cytometry)
Assess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry.
Western blotting to detect ATP13A2 and ATP13A3
Protocol to detect ATP13A2 and ATP13A3 via Western Blotting.
Microsomal membrane isolation from cell culture
Microsomal membrane isolation from cell culture
Cellular lipid uptake with flow cytometry readout
Cellular lipid uptake with flow cytometry readout
Cell surface biotinylation
Cell surface biotinylation
Western blotting
Western blotting
Astrocyte extraction from brain organoids V.2
Protocol for astrocyte extraction from brain organoids.
Evaluating endocytic rate in cells
Assess endocytic rate in cells using tagged transferrin (Alexa647) and flow cytometry readout.
Western blot protocol for detecting ATP10B in mouse/rat brain
Protocol for detection of ATP10B in rat and mouse brain tissue by Western blotting.
Lysosome proteolysis analysis with DQ-BSA
Protocol to analyze lysosomal proteolysis in astrocytes generated from iPSCs.
