Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
Cryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
Selective loss of Primary Cilia and Neurotrophic Signaling in G51D α-Synuclein Mice Highlights a Common Pathway to Parkinson’s Disease
G51D α-synuclein mice mimic disease symptoms, showing cilia loss in specific neurons and impaired neurotrophic signaling, contributing to disease progression. This highlights the role of ciliary dysfunction in Parkinson’s.
Robust analytical methods for bis(monoacylglycero)phosphate profiling in health and disease
BMP is a crucial phospholipid in lysosomes, impacting metabolic balance and linked to neurodegenerative diseases. LC-MS methods are detailed for accurate BMP analysis, utilizing structural differences and a new CLN5 function for profiling.
A TIRF microscopy-based assay to monitor LRRK2-dependent endo-lysosome exocytosis
Here we describe a method for monitoring CD63+ endo-lysosome exocytosis in hTERT-RPE cells stably expressing CD63-pHluorin.
pMX-Flag-GFP_SlC45A1
GFP-SLC45A1 plasmid.
pMX-Flag-GFP_PLEKHB1
GFP-PLEKHB1 plasmid
pMX-Flag-GFP_SlC45A1 mutant p.Arg210Trp
GFP-SLC45A1 plasmid with p.Arg210Trp mutation
pMX-Flag-GFP_TMEM256
GFP-TMEM256 plasmid
pMX-Flag-GFP_TMEM88B
GFP-TMEM88B plasmid
hENPP5-EGFP
GFP-ENPP5 plasmid human
hCBLN3-EGFP
GFP-CBLN3 plasmid
pcDNA-pDEST_TMEM192_BirA_Flag
TMEM192_BirA plasmid for lysosomal proximity labelling
hEDIL3-EGFP
GFP-Edil3 plasmid human
pcDNA-pDEST_BirA_Flag
BirA-only control plasmid
pcDNA-pDEST_BirA_Flag_SLC45A1
Slc45A1_BirA plasmid for SLC45A1 proximity labelling
