Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Leucine Rich Repeat Kinase 2: Pathways to Parkinson’s Disease
Research on LRRK2 in Parkinson's disease has advanced, revealing how mutations activate the kinase, phosphorylate Rab proteins, disrupt cell functions like Hedgehog signaling, and contribute to the disease. LRRK2 inhibitors show promise for therapy.
Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
Cryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
Leucine-rich repeat kinase 2 impairs the release sites of Parkinson’s disease vulnerable dopamine axons
Synaptic dysfunctions manifest early in Parkinson's disease. Research on LRRK2 mutations, using advanced genetic models, reveals their effects on neuronal synapses, primarily impacted by the disease, offering insights into potential early therapies.
Primary data associated with Figure 3–Figure Supplement 4 in doi: 10.1101/2022.04.25.489459
Raw immunoblotting data and tabular data for quantitation used to generate Figure 3–Figure Supplement 4 in doi: 10.1101/2022.04.25.489459 ("A Feed-forward Pathway Drives LRRK2 kinase Membrane Recruitment and Activation").
Binding of Rab29 to the LRRK2 Armadillo domain by Microscale Thermophoresis
Microscale thermophoresis is a powerful tool to measure the affinities of interactions between proteins. We present here our method for determining the binding of Rab29 GTPase to the LRRK2 N-terminal Armadillo domain.
Primary data associated with doi: 10.1042/BCJ20220161 (Impact of 100 LRRK2 variants linked to Parkinson’s Disease on kinase activity and microtubule binding)
Raw immunoblotting data; tabular data for quantifications; numerical data for in vitro assays; immunofluorescence images; Prism files for statistical analysis.
HEK293 TMEM115-3xHA GolgiTAG (CVCL_C8A6)
Cell line generated from HEK293 cells (Cat# CRL-1573, RRID:CVCL_0045) virally transfected with a construct expressing TMEM115-3xHA (GolgiTAG)
Role of autophagy pathway in Parkinson’s disease and related Genetic Neurological disorders
The authors provide a comprehensive overview of the general importance of autophagy in Parkinson’s disease (PD) and related disorders of the central nervous system (CNS).
Staining of cells with GolgiTracker for Golgi flow cytometry analysis
Method that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis.
Parkinson’s VPS35[D620N] mutation induces LRRK2-mediated lysosomal association of RILPL1 and TMEM55B
This study uncovers a pathway through which dysfunctional lysosomes resulting from the VPS35[D620N] mutation recruit and activate LRRK2 on the lysosomal surface, driving assembly of the RILPL1-TMEM55B complex.
Primary confocal microscopy data associated with the manuscript “Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B” (doi: 10.1101/2023.06.07.544051)
Confocal microscopy images, CellProfiler Excel files of Pearson’s coefficients between TMEM55B/RILPL1 or pRab10/RILPL1 in R1441C MEF or VPS35[D620N] MEF cells, and Prism files of graphed Pearson’s coefficients for each condition, as seen…
Crystal structure of a substrate-trapping variant of PPM1H phosphatase
Crystal structure of a substrate-trapping variant of PPM1H phosphatase (as reported in 10.15252/embr.202152675) deposited in the Protein Data Bank with code 7L4I.
Structure of wild-type PPM1H phosphatase at 3.1 Angstrom resolution
Structure of wild-type PPM1H phosphatase at 3.1 Angstrom resolution (as reported in 10.15252/embr.202152675) deposited in the Protein Data Bank with code 7KPR.
Untargeted metabolomics analysis for Golgi immunopurification (Golgi-IP)
This protocol provides details for analyzing GolgiIP metabolomics samples using liquid chromatography mass spectrometry (LC-MS) for polar metabolite profiling.
Primary data associated with the manuscript “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (2/3)
Primary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase."
