Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
Cryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
Copica, an open-source easy-to-use protein copy number mass spectrometry database
Advances in mass spectrometry allow profiling proteome with 10 ng material. Copica database aids in analyzing and comparing protein abundance in cells and tissues, highlighting specific proteins and enabling easy comparison of datasets.
CURTAIN, A WEB-BASED TOOL FOR DATA VISUALIZATION AND EXPLORATION OF MASS SPECTROMETRY-BASED PROTEOMICS
Curtain is a tool for sharing, visualizing, and analyzing mass spectrometry data, designed for non-experts. It allows easy data sharing via web links and displays differential analysis results in interactive plots.
CURTAIN-PTM, A WEB-BASED TOOL FOR DATA VISUALIZATION AND EXPLORATION OF MASS SPECTROMETRY-BASED PROTEOMICS (POST TRANSLATIONAL MODIFICATIONS)
Curtain is a tool for sharing, visualizing, and analyzing proteomic and PTM MS data. It allows easy sharing of data via web link, displays results in interactive plots, and enables analysis of protein groups and structure. Free and open-source.
Cactus
Cactus (10.5281/zenodo.7351087) manages session data for curtain and curtainptm backends, handling saving and loading operations efficiently.
Release Notes – ASAP CRN Cloud – Version 4.0.0
ASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New collections and individual datasets were added, enhancing PMDBS and Mouse RNA sequencing data.
Biochemical analysis of PINK1 activation and ubiquitination signaling upon GTPP treatment
Ubiquitinated proteins were enriched using Halo-m-DSK ubiquitin capture, and GFP-PINK1 complexes were isolated by IP, followed by SDS–PAGE and immunoblotting to assess PINK1 activation and downstream ubiquitination signaling upon GTPP treatment
Tabular data from Mitochondrial Proteostasis Cryo-ET Analysis
Tabular output data from the Mitochondrial Proteostasis Cryo-ET Analysis as demonstrated in Ehses et al., 2026.
Molecular dynamics flexible fitting output of mHsp60 models into in situ density maps
MDFF simulations were performed to evaluate how well atomic models of mHsp60 single rings in different nucleotide states fit the in situ subtomogram averaging density maps by RMSD-based assessment of model–map agreement.
Fluorescence microscopy analysis of PINK1 localization upon GTPP treatment
PINK1-GFP Knock-in cells were stained with the mitochondrial membrane potential–dependent dye MitoSpy Red CMXRos and nuclear DNA dye Hoechst 33342 following treatment with 10µM G-TPP over multiple time points.
ADP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ADP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 half-football complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 half-football complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 bullet complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 bullet complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
