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Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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FISH CODETECTION
DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
Solutions used for FISH Codetection
Protocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
MANUAL CELL COUNTING
MANUAL CELL COUNTING
Stereotactic injection of viral vectors
Here we describe stereotactic injection of AAV into ventral tegmental area (VTA) of mice.
Combined RNAscope and immunohistochemistry labeling of fresh mouse midbrain tissue
Here we describe labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for the gene Slc17a6 (VGLUT2) and virally-expressed histone-tagged GFP.
Protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
Step by step procedure for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice.
Stereotactic Viral Injection into Subthalamic Nucleus in Mice
This protocol describes the surgical procedure of injecting AAV viruses into the subthalamic nucleus of a mouse. It contains information regarding the stereotactic setup, surgery preparation, anesthesia, craniotomy, injection, and recovery.
Handling and behavioral training protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
This protocol details the habituation of animals and behavioral training of animals for in vivo electrophysiology or optogenetic experiments.
Endosome isolation
Subcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
Transcardiac Perfusion of Mouse for Brain Tissue
Transcardiac perfusion is a method used for clearing blood and preserving the mouse brain for immunostaining.
Create Mask for Axonal Quantification Analysis with FIJI
This protocol describes how to create a quantified mask from an image of fluorescence-tagged axonal projections using FIJI/ImageJ software.
Catalepsy test (Bar test)
The catalepsy test (bar test) was developed to test motor coordination and motor impairments.
Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
This behavior is used to assess fine motor movements in a mouse Parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
Immunofluorescent labeling of cultured cells
This protocol describes the process of Paraformaldehyde fixation [PFA-fixation] and immunofluorescent-labeling of cultured cells.
Genotyping mice from ear clips
This protocol describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjusted.