Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Theme
DOPA pheomelanin is increased in nigral neuromelanin of Parkinson’s disease
Neuromelanin in the substantia nigra may be a key factor contributing to dopaminergic neuron vulnerability in Parkinson’s disease. Here, the authors investigated the relative composition and specific roles of pheomelanin and eumelanin in PD.
Cryosectioning Mouse Brain
This protocol details the cryosectioning of the mouse brain.
Post mortem human substantial nigra TH staining
This protocol is standardized for postmortem (frozen) SN tissue from pathologically diagnosed PD patients and control individuals for Immunofluorescence staining.
Unilateral intranigral AAV alpha synuclein mouse model
As some protocols have not been made public and are only shared within the network, you may need to login to the protocols.io to view the link. Don’t have a login? Use this <a…
Determination of NM Concentration
This is the protocol for determining neuromelanin concentration and data.
Immunofluorescent staining for neuronal marker MAP2
This is the protocol for immunofluorescent staining for neuronal marker MAP2.
Human neuroblastoma cell line SH-SY5Y culturing
This is the brief protocol for seeding and culturing human neuroblastoma SH-SY5Y cells.
Parkinson’s disease and cancer: a systematic review and meta-analysis of over 17 million participants
The authors examined risk association between Parkinson’s disease and cancer using data from 63 publications. With the exception of melanoma, the authors found that the risk association of Parkinson’s disease and cancer was inversely related.
Striatal dopamine measurement through HPLC
Protocol for striatal dopamine measurement in mouse brain
Cell viability assessment
This is the protocol for assessing cell viability using a MTS cell proliferation assay kit.
Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK
Activating V600E in v-Raf murine sarcoma viral oncogene homolog B (BRAF) is a common driver mutation in cancers of multiple tissue origins, including melanoma and glioma. BRAFV600E has also been implicated in neurodegeneration. The present study…
Chemical degradation and determination of pheomelanin and eumelanin markers
This is protocol involving chemical oxidation and reduction methods followed by high performance liquid chromatography (HPLC) detection of markers for pheomelanin and eumelanin.
Peripheral MC1R activation modulates immune responses and confers neuroprotection in a mouse model of Parkinson’s disease
Dataset corresponding to the article, "Peripheral MC1R activation modulates immune responses and confers neuroprotection in a mouse model of Parkinson's disease."
Peripheral MC1R Activation Modulates Immune Responses and is Neuroprotective in a Mouse Model of Parkinson’s Disease
Preprint: The present study investigates the impact of NDP-MSH, a synthetic melanocortin receptor (MCR) agonist that does not cross BBB, on the immune system and the nigrostriatal dopaminergic system in mouse model of PD.
LC/MS analysis of plasma samples from PPMI
Plasma samples from PPMI were analyzed by liquid chromatography with mass spectrometry (LC/MS) for a variety of metabolites (including piperine) and lipids as interrelated markers of Parkinson’s disease and its pathophysiology.
