Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Release Notes – ASAP CRN Cloud – Version 4.0.0
ASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New collections and individual datasets were added, enhancing PMDBS and Mouse RNA sequencing data.
Dopamine release from Parkinson’s patient-derived neurons is disrupted due to impaired synaptic vesicle loading
Human Parkinson's patient neurons with SNCA-triplication mutation exhibit reduced dopamine release due deficits in dopamine loading and handling.
pCMV-DEAD XPACK-DNAJC5 (L115R)
Mammalian expression of DEAD XPACK-DNAJC5 (L115R)
Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
This protocol describes the maintenance and expansion of iPSCs in the adherent culture via single-cell passaging.
pLenti-CMV-DNAJC5 (L115R)
Mammalian expression of DNAJC5 (L115R)
Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.
Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”
The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
This protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
This protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
pCMV-XPACK-DNAJC5 (L115R)- HaloTag
Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag
pCMV-DNAJC5 (L115R)-HaloTag
Mammalian expression of DNAJC5 (L115R)-HaloTag
pCMV-DNAJC5-ΔC40 (D151–198)
Mammalian expression of DNAJC5-ΔC40 (Δ151-198)
Differentiation of human medium spiny neurons (MSNs) from induced pluripotent stem cells (iPSCs)
This protocol generates human medium spiny neurons (MSNs) from induced human pluripotent stem cells (iPSCs).
pCMV-DNAJC5-ΔC30 (D169–198)
Mammalian expression of DNAJC5-ΔC30 (Δ169-198)
pCMV-pOTC-GFP
Plasmid: Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase.
