This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
results for ""
Sort
Filter & Sort
-
Output Type
-
Program
-
CRN Team Name
-
Theme
Determining the effects of mecamylamine in the mouse striatum using a Conditioned Preference Place (CPP) paradigm
Protocol tests mecamylamine effects in mouse striatum using CPP paradigm to measure preference for drug-associated environment.
Intracranial injections of viral vectors in mouse midbrain and striatum
Protocol for injecting viral vectors in mouse midbrain and striatum is provided.
Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings
Protocol labels ChAT for cholinergic interneurons and TH for dopaminergic neurons in mouse brain slices. Immunofluorescence used post ex vivo voltammetry recordings on 300-µm thick slices.
In vivo voltammetry and fiber photometry in the mouse striatum
Protocol measures dopamine and calcium levels in striatal dopaminergic axons in vivo via voltammetry and fiber photometry in response to electrical stimulation.
Wide-field imaging of voltage sensors expressed in ex vivo mouse brain slices
This protocol describes how to perform wide-field imaging of voltage sensors using high frame rates (660 Hz minimum every 2.5 minutes) in mouse midbrain using ex vivo brain slices.
Imaging axonal calcium dynamics in ex vivo mouse brain slices
Protocol for imaging calcium dynamics in striatal dopaminergic axons in ex vivo mouse brain slices using GCaMP6f in DAT-Cre:Ai95D mice. Calcium transients were observed in response to single and train electrical stimuli.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices following electrical or optogenetic stimulations
This protocol is to assess changes in extracellular dopamine concentration following electrical or optogenetic stimulations in ex vivo mouse brain slices.
Datasets and Key Resources Table used in Zang, Y. et al. (2025) “An axonal brake on striatal dopamine output by cholinergic interneurons”
This repository contains a 1) Key Resources Table with details on key resources and the persistent identifiers for protocols and code used and generated in this study; 2) Source Data Folder, and 3) Supplementary Data Folder.
Custom MATLAB scripts related to Zhang, Y. et al (2025) “An axonal brake on striatal dopamine output by cholinergic interneurons”
MATLAB scripts written by Yan-Feng Zhang to predict how nicotinic receptors impact on dopamine transient in vivo during the dynamic tonic and multiphasic activity in cholinergic interneurons.
pCMV-DEAD XPACK-DNAJC5 (L115R)
Mammalian expression of DEAD XPACK-DNAJC5 (L115R)
Expansion and maintenance of human induced pluripotent stem cells (iPSCs)
This protocol describes the maintenance and expansion of iPSCs in the adherent culture via single-cell passaging.
pLenti-CMV-DNAJC5 (L115R)
Mammalian expression of DNAJC5 (L115R)
Datasets and Key Resources Table used in Do, Quyen B. et al. (2023) “Early striatal hyperexcitability in an in vitro human striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation”
The files found here are associated to Do, Q. et al (2023) study and contain: (a) all source and supplementary data, (b) Key Resources Table, and (c) list of primers.
Early deficits in an in vitro striatal microcircuit model carrying the Parkinson’s GBA-N370S mutation
The results highlight the unique utility of modeling striatal neurons in a modular and highly physiological circuit, which is essential to reveal mechanistic insights of the loss of electrical functional integrity in the striata of GBA1 PD patients.
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
This protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.