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Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Defect in hematopoiesis and embryonic lethality at midgestation of Vps13a/Vps13c double knockout mice
VPS13 proteins facilitate lipid transfer at membrane contact sites. VPS13A and VPS13C double knockout causes embryonic death due to impaired erythropoiesis and innate immunity activation.
The bridge-like lipid transport protein VPS13C/PARK23 mediates ER-lysosome contacts upon lysosome damage
Genetic studies link lysosome dysfunction to Parkinson's disease. VPS13C, a PD gene, relocates to damaged lysosomes, aiding in membrane repair. This process involves Rab7 and suggests early lipid transport as a protective response.
ATG2A engages Rab1a and ARFGAP1 positive membranes during autophagosome biogenesis
ATG2A aids in autophagosome formation by localizing to extra-Golgi ARFGAP1 puncta with Rab1 during early stages. Rab1 is crucial for autophagy, and disruptions in autophagosome formation lead to accumulation of ARFGAP1 and Rab1a at ectopic sites.
iPSC differentiation into Macrophages
This protocol describes iPSC differentiation into macrophages.
Nuclear cytoplasmic fractionation
This protocol describes nuclear cytoplasmic fractionation.
VPS13D(∆ATG2-C/PH)^EGFP
Plasmid: VPS13D with deleted DHPH domain.
DQ-BSA quantification
This protocol describes DQ-BSA quantification.
Generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
This protocol is to help with generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
Membrane remodeling properties of the Parkinson’s disease protein LRRK2
Preprint: The authors examine how purified LRRK2 directly binds acidic lipid bilayers in a cell-free system and can deform them into narrow tubules in a guanylnucleotide-dependent but ATP-independent way.
Surface protein biotinylation
This protocol describes surface protein labeling with biotin using EZ-Link Sulfo-NHSLC-Biotin. This chemical reacts with primary amines such as lysine but does not permeate cell membranes because of the charge. Thus, it only biotinylates surface…
RNA isolation and qRT-PCR
This protocol describes the RNA isolation from cultured cells and quantitative RT PCR.
SHIP164 is a chorein motif lipid transfer protein that controls endosome-Golgi membrane traffic
Cellular membranes differ in protein and lipid composition as well as in the protein-lipid ratio. Thus, progression of membranous organelles along traffic routes requires mechanisms to control bilayer lipid chemistry and their abundance relative to…
Protein purification
This protocol details how to purify 3xFLAG-SHIP164Δ901-1099
DQ-BSA assay
This protocol describes the DQ BSA assay for measuring the lysosomal proteolytic activity.
Cell culture, transfection, and imaging
This protocol describes general procedures for culturing HeLa cells, transient transfection, and imaging using an Andor Dragonfly spinning disk confocal system.