Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
-
Output Type
-
Program
-
CRN Team Name
-
Theme
iPSC differentiation into Macrophages
This protocol describes iPSC differentiation into macrophages.
Nuclear cytoplasmic fractionation
This protocol describes nuclear cytoplasmic fractionation.
VPS13D(∆ATG2-C/PH)^EGFP
Plasmid: VPS13D with deleted DHPH domain.
DQ-BSA quantification
This protocol describes DQ-BSA quantification.
Membrane remodeling properties of the Parkinson’s disease protein LRRK2
Preprint: The authors examine how purified LRRK2 directly binds acidic lipid bilayers in a cell-free system and can deform them into narrow tubules in a guanylnucleotide-dependent but ATP-independent way.
Generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
This protocol is to help with generation of knock-in and knock-out CRISPR/Cas9 editing in mammalian cells
SHIP164 is a chorein motif lipid transfer protein that controls endosome-Golgi membrane traffic
Cellular membranes differ in protein and lipid composition as well as in the protein-lipid ratio. Thus, progression of membranous organelles along traffic routes requires mechanisms to control bilayer lipid chemistry and their abundance relative to…
Surface protein biotinylation
This protocol describes surface protein labeling with biotin using EZ-Link Sulfo-NHSLC-Biotin. This chemical reacts with primary amines such as lysine but does not permeate cell membranes because of the charge. Thus, it only biotinylates surface…
Protein purification
This protocol details how to purify 3xFLAG-SHIP164Δ901-1099
RNA isolation and qRT-PCR
This protocol describes the RNA isolation from cultured cells and quantitative RT PCR.
DQ-BSA assay
This protocol describes the DQ BSA assay for measuring the lysosomal proteolytic activity.
Cell culture, transfection, and imaging
This protocol describes general procedures for culturing HeLa cells, transient transfection, and imaging using an Andor Dragonfly spinning disk confocal system.
RBG Motif Bridge-Like Lipid Transport Proteins: Structure, Functions, and Open Questions
The life of eukaryotic cells requires the transport of lipids between membranes, which are separated by the aqueous environment of the cytosol. Vesicle-mediated traffic along the secretory and endocytic pathways and lipid transfer proteins (LTPs)…
Subtomogram averaging and classification
This protocol describes the procedure of subtomogram averaging and 3D classification of VPS13C rod-shaped densities inside cryotomograms of mammalian cells. Sub-tomogram averaging package i3 was used for 3D alignment and classification.
Regular maintenance of human pluripotent stem cells
This protocol describes the regular maintenance and passaging human iPSCs.
