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Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Leveraging animal models to understand non-motor symptoms of Parkinson’s disease
Parkinson's disease diagnosis focuses on motor symptoms, but non-motor symptoms like cognitive issues and sleep disorders significantly affect patients. Research on non-motor signs in animals aims to improve understanding of the disease.
Preparation of Acute Brain Slices
This protocol describes the steps for preparing acute brain slices.
Postsynaptic adaptations in direct pathway muscarinic M4-receptor signaling follow the temporal and regional pattern of dopaminergic degeneration
Imbalances in dorsal striatum output in Parkinson's disease are driven by dopamine loss and disrupted acetylcholine signaling. These changes occur in response to dopamine loss, affecting M4 receptors in striatal neurons, crucial for PD progression.
Stereotaxic Surgery
This protocol describes the steps for performing mouse stereotaxic surgery. It is applicable to intracranial injections of viruses and drugs.
6-OHDA mouse model of Parkinson’s disease
Protocol for creating a Parkinson's disease mouse model using 6-OHDA involves injecting different doses into the brain to induce varying levels of dopaminergic damage.
Levodopa treatment for low-dose 6-OHDA-treated mice
This protocol describes a one-week levodopa treatment for mice partially depleted of dopamine.
Ex Vivo Electrophysiology – Ford Lab
This protocol describes the steps to perform whole-cell electrophysiology recordings in acute brain slices.
Electrophysiology and 2-photon imaging of Ca+2-transients
Protocol for imaging dendritic calcium transients using 2-photon microscopy with a Ti:Sapphire laser. Fluorescence collected through objective, dichroic mirror, and filters, then detected by a GaAsP PMT. Output digitized for analysis.
Motor Behavioral Assessment
Protocol outlines motor behavior tests for Parkinson's disease in mice using open field, rotarod, cylinder, and balance beam tests. Mice were habituated for 30-40 min before tests conducted 3-4 weeks post-injections.
Data for Jain et al Adaptor Protein-3 Produces Synaptic Vesicles that Release Phasic Dopamine
Primary data for Jain et al "Adaptor protein-3 produces synaptic vesicles that release phasic dopamine" (Proc Natl Acad Sci. 2023; 120(42):e2309843120. doi: 10.1073/pnas.2309843120)
Slow Release Pellet Implantation
This protocol describes how to subcutaneously implant slow-release pellets into mice.
Striatal Punch Collection
This protocol describes how to collect striatal punches from fresh frozen mouse brain tissue for HPLC analysis.
Mouse Behavior – Open Field and T-Maze
This protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
Live Imaging of Primary Mouse Neuron Cultures
This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging described involves labeling of dopamine neurons with a…
In Vivo Electrophysiology (Mouse)
This protocol describes the in vivo electrophysiology method for recording neuronal activity in mice.