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Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Mouse Whole Gut Transit Time
Protocol used in the Gradinaru lab for obtaining whole gut transit time for mice
Mouse Beam Crossing
Protocol used in the Gradinaru lab for mouse beam test
Mouse perfusion
Protocol used in the Gradinaru lab for perfusing mice
Coronal cryosectioning of mouse brains
Protocol used in the Gradinaru lab for coronal cryosectioning of mouse brain tissue with a cryostat
Immunostaining Mouse Tissue
Protocol used in the Gradinaru lab for immunohistochemistry to analyze protein expression in the mouse brain
RNA extraction from cells
Protocol used in the Gradinaru lab for RNA extraction from cells
RNA extraction from organoids
Protocol used in the Gradinaru lab for RNA extraction from organoids
Copy of RNA extraction from mouse tissue
Protocol used in the Gradinaru lab for RNA extraction from mouse tissue
Genetically encoded self-assembling synuclein for tunable Parkinsonian pathology in vitro and in vivo
This paper describes the self-assembling synuclein (SAS) system, a genetically-encoded tool for inducing temporally-controllable, tunable PD pathology in a wide range of in vitro and in vitro models.
Transfection of HEK cells with Lipofectamine 3000
Protocol used in the Gradinaru lab to transfect HEK cells with lipofectamine 3000
Immunostaining Cells
Protocol used in the Gradinaru lab for antibody staining of cells
Western Blot with iBind System
Protocol used in the Gradinaru lab for western blot using the iBind Automated Western System
Primary Neuron Extraction
Protocol used in the Gradinaru lab to extract primary neurons from mouse on day E17
iPSC neuron development
Protocol used in the Gradinaru lab to develop iPSC neurons
Mouse Open Field
Protocol used in the Gradinaru lab for general locomotion in rodents using open field arenas and Ethovision video tracking