Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Cryo-electron tomography of thinned synapses
Protocol for cryo-ET of thinned synapses in rat neuron cultures is detailed. Cryo-FIB milling creates thin samples for high-resolution imaging. Synapse targeting with and without cryo-FLM correlation is explained.
Single particle analysis of α-Synuclein fibrils
Aggregates of α-Synuclein are linked to Parkinson’s, multiple system atrophy, and Lewy body dementia. This protocol outlines near-atomic resolution structures of these amyloid fibrils using cryo-EM single particle analysis.
Preparing primary sandwich hippocampal neuron cultures for cryo-electron tomography
Sandwich hippocampal neuron cultures have low cell density and few glial cells, making them ideal for targeting neurons for cryo-electron tomography.
Single particle analysis datasets of in vitro grown aSyn filaments
Dataset includes cryo-EM micrographs of a-Synuclein fibrils with WT and mutant forms (G14R, K58N) found in patients.
Tomogram of aggregate in AgDD-sfGFP-expressing HEK293 cell 10 min post aggregation induction
AgDD protein aggregation can be controlled for cellular studies. A cryo-electron tomogram shows an AgDD aggregate in a HEK293 cell 10 min post-aggregation induction.
Tomogram of aggregate in AgDD-sfGFP-expressing HEK293 cell 6 h post aggregation induction
AgDD protein aggregation in HEK293 cells can be temporally controlled for studying cellular effects. A cryo-electron tomogram shows AgDD aggregate formation 6h post-induction.
Cryo-EM map of in vitro grown G14R alpha-synuclein fibrils
3.0 A resolution cryo-EM map obtained from recombinant a-synuclein fibrils carrying the G14R mutation recently discovered in a patient of a complex neurological disease.
Cryo-EM map of in vitro grown WT alpha-synuclein fibrils
Cryo-EM density map of recombinant WT a-synuclein fibrils at 2.7 A resolution.
Cryo-EM map of recombinant K58N alpha-synuclein fibrils
Cryo-EM map obtained from recombinant a-synuclein fibrils carrying the K58N mutation recently discovered in a patient of Parkinson's disease.
A Novel α-Synuclein K58N Missense Variant in a Patient with Parkinson’s Disease
15% of cases of PD are linked to mutations in the SNCA gene. A novel K58N mutation in a patient showed typical PD symptoms, genetic testing, and biophysical studies revealing structural effects and abnormal cellular behavior of SNCA.
Proteome Landscapes Decode Organelle Vulnerabilities in cortical and dopaminergic-like induced neurons Across Lysosomal Storage Disorders
Lysosomal Storage Disorders impact lysosomal function and can be linked to Parkinson's Disease risk. LSD mutant cells show varied endolysosomal proteomes, with specific disruptions in neuronal cells.
Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
Cryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
CRISPR tagging of the TMEM192 gene with 3xHA in H9 ES AAVS-NGN2 cells for Lyso-IP
Protocol for adding a 3X HA tag to TMEM192 gene in human cells using CAS9 and recombination template is described for H9 ES AAVS1-NGN2 cells.
