Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Regulation of the proteasome 20S core particle by the Parkinsonism-associated Proteins FBXO7 and PI31
Genetic forms of PD are associated with variants in FBXO7 and PI31. Combining biochemical and structural approaches, we describe the proteasome interaction of these proteins and map disease variants to disruption of proteasome interaction.
Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress
Cryo-ET showed protein aggregates, altered cristae, and reduced ribosome complexes in stressed mitochondria. Mitochondrial Hsp60 undergoes conformational changes to aid in protein folding, shedding light on mitochondrial proteostasis mechanisms.
HeLa PINK1-GFP knock-in cell line
A HeLa cell line expressing PINK1 fused to GFP from the endogenous locus was generated using CRISPR–Cas9 nickase–mediated genome editing with homology-directed repair. GFP-positive clones were isolated by fluorescence-activated cell sorting.
Biochemical analysis of PINK1 activation and ubiquitination signaling upon GTPP treatment
Ubiquitinated proteins were enriched using Halo-m-DSK ubiquitin capture, and GFP-PINK1 complexes were isolated by IP, followed by SDS–PAGE and immunoblotting to assess PINK1 activation and downstream ubiquitination signaling upon GTPP treatment
Tabular data from Mitochondrial Proteostasis Cryo-ET Analysis
Tabular output data from the Mitochondrial Proteostasis Cryo-ET Analysis as demonstrated in Ehses et al., 2026.
Molecular dynamics flexible fitting output of mHsp60 models into in situ density maps
MDFF simulations were performed to evaluate how well atomic models of mHsp60 single rings in different nucleotide states fit the in situ subtomogram averaging density maps by RMSD-based assessment of model–map agreement.
Fluorescence microscopy analysis of PINK1 localization upon GTPP treatment
PINK1-GFP Knock-in cells were stained with the mitochondrial membrane potential–dependent dye MitoSpy Red CMXRos and nuclear DNA dye Hoechst 33342 following treatment with 10µM G-TPP over multiple time points.
ADP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ADP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 double-ring complex (C7), SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 single-ring complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 single-ring complex, SPA reconstruction, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 half-football complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 half-football complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 bullet complex (C7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 bullet complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
ATP-bound human mtHsp60:mtHsp10 football complex (D7), SPA reconstruction
Cryo-EM SPA density-map of a ATP-bound human mtHsp60:mtHsp10 football complex, from recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP.
Mitochondrial Proteostasis Cryo-ET Analysis
Collection of scripts used for the analysis of cryo-ET data in Ehses et al., 2026. The code, written in MATLAB 2019b, includes pipelines for tomogram pre-processing and reconstruction as well as particle quantification.
SPA dataset of in vitro human mHsp60 assemblies
Dataset used for Single-particle cryo-EM analysis of recombinant human mHsp60 and mHsp10 complexes, reconstituted in vitro in the presence of ATP. Purified proteins were incubated under defined conditions and vitrified at multiple time points.
