Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
-
Output Type
-
Program
-
CRN Team Name
-
Theme
A RAB7A Phosphoswitch Coordinates Rubicon Homology Protein Regulation of PINK1/Parkin-Dependent Mitophagy
Published: Structural and functional data support a model in which the TBK1-dependent phosphorylation of RAB7A serves as a switch, promoting mitophagy by relieving Rubicon inhibition and favoring Pacer activation. View original preprint.
EXPRESSION AND PURIFICATION OF HUMAN NEMO (GST-GFP-NEMO)
This protocol describes how to express and purify human NEMO (IKK-γ) tagged N-terminally with GST and EGFP.
pGST2-NDP52
Plasmid for the purification of recombinant GST-tagged NDP52 protein.
pCAG-MBP-ATG9-V836A
Plasmid for expression of human ATG9-V836A mutant in mammalian cells
CoxII degradation assay to assess mitophagy
This protocol details the procedure to assess mitophagy by analyzing COXII degradation via Western blotting.
Microscopy-based bead assay
This protocol describes the procedure to perform microscopy-based bead assay.
Generation of ATG3 KO Hela cells stably expressing HaloTag-LC3B
This protocol details generation of ATG3 KO HeLa cells stably expressing HaloTag-LC3B.
2BT-His-TEV-cs-ATG3_Y210A
DISCONTINUED - Plasmid for bacterial Expression of human ATG3 Y210A.
RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors
Gain-of-function mutations in LRRK2 gene lead to by increasing phosphorylation of RAB GTPases. Over-phosphorylation of RAB3A disrupts synaptic vesicle transport in human neurons, altering synaptic protein distribution and likely contributing to PD.
pHTN-Halo-TEV-ATG3_H266Q
Plasmid for Mammalian Expression of human ATG3 H266Q.
pHTN-Halo-TEV-ATG3_C264S
Plasmid for Mammalian Expression of human ATG3 C264S.
pCAG-MBP-ATG9-P834A
Plasmid for expression of human ATG9-P834A mutant in mammalian cells.
CRISPR/Cas9 generation of knock-out iPSCs
This protocol describes RNP-based CRISPR/Cas9 gene-editing to generate knock-out iPSCs.
pBMN_HA-TBK1 (kinase dead – D135N)
Plasmid: Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997).
