This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
results for ""
Sort
Filter & Sort
-
Output Type
-
Program
-
CRN Team Name
-
Theme
Leveraging animal models to understand non-motor symptoms of Parkinson’s disease
Parkinson's disease diagnosis focuses on motor symptoms, but non-motor symptoms like cognitive issues and sleep disorders significantly affect patients. Research on non-motor signs in animals aims to improve understanding of the disease.
RNAScope in situ hybridization (ISH) multiplex fluorescent
Instructions to perform ISH using RNAscope kit from ACDBio company optimized for frozen mouse brain and nodose ganglia sections.
Accelerated Ovarian Failure AOF mouse model: a model of transitional menopause
The novel model of AOF successfully replicates the human peri- and post-menopause stages, including erratic estrous cycles and hormone fluctuations, followed by undetectable estrogen levels.
Fluorescent Immunolabelling for endogenous mouse LRRK2
This protocol is used to label endogenous LRRK2 in mouse. It has been edited from West et al., 2014. Protocol optimized for fresh fixed brain tissue sectioned at 40 um.
GI Transit Assay
This test is used to check for possible signs of constipation and alteration in gut transit.
Cylinder Test
The Cylinder test is used to evaluate locomotor asymmetry in rodent models.
Fluorescent Immunolabelling for endogenous ChAT in mice striatum
This immunohistochemistry protocol has been designed and adjusted specifically for ChAT immunolabelling in the mice striatum.
Glucose Tolerance Test
The glucose tolerance test is performed to determine how quickly the glucose is cleared from the blood. It is used to test for diabetes or insulin resistance.
Mouse Ovariectomy
This protocol describes the steps to perform an ovariectomy in a mouse. This procedure is used to induce menopause.
Challenging Beam Test
The challenging beam test is used to measure motor performance, coordination, and balance.
Wire Hang Test
This behavioral test is designed to assess early motor deficits associated with muscle and grip strength.
Fecal Water Content Assay
This test is used to check for signs of constipation.
AAV Purification Protocol with Iodixanol gradient
Protocol used in the Kaplitt and Marongiu labs to purify AAVs.
Fluorescent Immunolabelling for Alpha-Synuclein in neuronal primary culture (Testing PFF toxicity)
This protocol is designed to perform fluorescent immunolabelling on neuronal primary culture after PFF incubation. The labelling of phosphorylated alpha-synuclein is considered as a marker of the PFF toxicity and should be done to validate the PFFs.
Pole Test – mice
This test assesses motor coordination in mice by assessing a mouse's ability to turn and descend on a vertical pole.