Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions
The authors developed an iPSC toolbox utilizing piggyBac-based or targeted transgenes to rapidly induce CNS cells with concomitant expression of aggregation-prone proteins.
Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation
A STAR protocol that describes how to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis
Generation of the iPSC line FINi002-A from a male Parkinson’s disease patient carrying compound heterozygous mutations in the PRKN gene
Generation of the iPSC line FINi002-A from a male Parkinson’s disease patient carrying compound heterozygous mutations in the PRKN gene. Primary fibroblasts from a skin biopsy of a male PD patient were reprogrammed using transiently present…
The pathogenesis of Parkinson’s disease
This review is the second in a Series of three papers about Parkinson’s disease published in the Lancet. Parkinson’s disease is a progressive neurodegenerative condition associated with the deposition of aggregated α-synuclein. Insights…
Free floating immunofluorescence protocol on mouse brain sections for tau pathology
This protocol describes the process of immunofluorescently labelling tau and phospho-tau in athymic mouse brains that have been grafted with human-disease derived iPSC's. This protocol can be adapted for other tissue types and antibody pairings.
Preparation of human iPSC-derived cortical neuronal progenitors for transplantation into the rodent brain
This protocol describes how we prepare human iPSC-derived cells, that have been differentiated into cortical neuronal progenitors, for transplantation into the brain of immunocompromised athymic mice. Neuronal progenitors mature within the mouse…
Dopamine and cortical iPSC-derived neurons with different Parkinsonian mutations show variation in lysosomal and mitochondrial dysfunction: implications for protein deposition versus selective cell loss
Comparison of dopamine neurons identified lysosomal and mitochondrial dysfunction, as well as increased tau deposition, alpha-synuclein phosphorylation and decreased glucocerebrosidase activity.
Transgenic A53T mice have astrocytic a-synuclein aggregates in dopamine and striatal regions
Using fluorescent immunohistochemistry, astrocyte subtypes were identified and aSyn burden quantified.
