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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
3′ mRNA-sequencing
Protocol for 3′ mRNA sequencing of mouse gastrointestinal tract tissues.
Dataset
A prebiotic diet modulates microglia response and motor deficits in α-synuclein overexpressing mice (Dataset)
These studies uncover a novel microglia-dependent interaction between diet and motor symptoms in mice, findings that may have implications for neuroinflammation and PD.
Teams
Article
A prebiotic diet modulates microglial states and motor deficits in α-synuclein overexpressing mice
Publication: The gut microbiome is altered in PD and may impact motor and GI symptoms as indicated by animal studies, although mechanisms of gut-brain interactions remain incompletely defined. Here, the authors investigated whether a fiber-rich diet influences microglial function in α-synuclein overexpressing (ASO) mice, a preclinical model with PD-like symptoms and pathology. View original preprint.
Teams
Dataset
A single-cell atlas of cell types, states, and other transcriptional patterns from nine regions of the adult mouse brain
We report 690K single-cell transcriptomes from nine different brain regions from adult mice (Frontal and Posterior Cortex, Hippocampus, Thalamus, Cerebellum, Striatum, Globus Pallidus externus/Nucleus Basalis, Entopeduncular / Subthalamic Nuclei, & Substantia Nigra / Ventral Tegmental Area).
Teams
Protocol
Activation Induced Marker (AIM) Staining Protocol
This protocol details about activation induced marker staining.
Teams
Article
Alpha-synuclein overexpression can drive microbiome dysbiosis in mice
Growing evidence indicates that persons living with Parkinson’s disease (PD) have a unique composition of indigenous gut microbes. Here, the authors used a transgenic mouse strain, which overexpress wild-type human α-syn to test how the gut microbiome composition responds in this model of PD pathology during aging.
Dataset
Axonal and somatodendritic proteomes of dopamine neurons in the mouse brain
The elaborate cytoarchitecture of DA neurons is embedded within complex brain tissues, making it difficult to access the DA neuronal proteome using conventional methods. This mass spectrometry proteomics dataset is a part of “Subcellular proteomics of dopamine neurons in the mouse brain” (Hobson et al., 2022).
Teams
Article
Border-associated macrophages mediate the neuroinflammatory response in an alpha-synuclein model of Parkinson disease
Published: The authors explored the role of resident macrophages in alpha-synuclein-mediated neuroinflammation. They found that border-associated macrophages (BAMs) play a key role in alpha-synuclein induced neuroinflammation where BAMs play a role in immune cell recruitment, infiltration, and antigen presentation. View original preprint.
Teams
Article
Central and Peripheral Inflammation: Connecting the Immune Responses of Parkinson’s Disease
Review: Authors highlight the important work being done that implicates central and peripheral inflammation in playing a role in PD. They also discuss how these two distant inflammations appear related and how that may be mediated by autoantigenic responses to α-synuclein.
Teams
Code
Code for integrated multi-cohort analysis of the Parkinson’s disease gut metagenome
Detailed scripts to reproduce all analysis and data visualization for the paper, “Integrated multi-cohort analysis of the Parkinson’s disease gut metagenome,” including an interactive shiny R app for exploration of metagenomic features of interest in the data sets.
Protocol
Colonic migrating motor complexes
Protocol for ex vivo assay of colonic migrating motor complexes (CMMCs) in mouse colons.
Dataset
Datasets associated with “Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology”
This RNA data set corresponds to the preprint: Neuronal Activation of the Gastrointestinal Tract Shapes the Gut Environment in Mice (Yoo et al., 2021).
Article
Dopamine transporter and synaptic vesicle sorting defects initiate auxilin-linked Parkinson’s disease
Published: Auxilin helps in recycling of synaptic vesicles to facilitate neurotransmission and loss of auxilin is associated with PD. The authors show auxilin knockout mice exhibit typical PD pathology, dopamine transport is disrupted due to slower dopamine reuptake kinetics, and that macroautophagy and defective synaptic vesicle sorting contributes to dopamine dyshomeostasis. View original preprint.
Dataset
ENS quantification
Labelled enteric neurons in living mice with recombinant adeno-associated viruses (rAAVs) expressing fluorescent proteins, and used tissue clearing techniques to enhance visualization of intact GI tissue.
Teams