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Output Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

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DAB ANTIBODY (IHC) STAINING PROTOCOL

This is the basic protocol for antibody staining of formalin fixed paraffin embedded (FFPE) tissue.

Program: Collaborative Research Network
Team:
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Pole Test

The protocol describes the pole test for assessing motor coordination and balance in rodents.

Program: Collaborative Research Network
Team:
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Open Field

This protocol details about Open Field procedure.

Program: Collaborative Research Network
Team:
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INTRAMUSCULAR PFF INJECTION

Protocol outlines intramuscular PFF injection in mice.

Program: Collaborative Research Network
Team:
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Loss of tau expression attenuates neurodegeneration associated with α-synucleinopathy

The authors examine the role of tau in the onset and progression of αS pathology using a transgenic mouse model of α-synucleinopathy lacking mouse tau expression.

Program: Collaborative Research Network
Team:
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Cx3cr1-cre;Ercc1-/fl mice

Microglia may experience higher levels of DNA damage and potential senescence due to internal factors.

Program: Collaborative Research Network
Team:
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Mouse Perfusion

The protocol outlines the process of mouse perfusion.

Program: Collaborative Research Network
Team:
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ROTAROD PROTOCOL

Protocol detailing the rotarod test procedure for research purposes.

Program: Collaborative Research Network
Team:
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ASAP CRN Cloud Release Notes – Version 3.0.0

Summary: Release notes for version 3.0.0 of CRN Cloud platform, released on September 30, 2025, detailing latest updates and enhancements.

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Barnes et al (Preprint-03/24) – Datasets

Datasets connected to Barnes et al can be found in their preprint publication at https://doi.org/10.21203/rs.3.rs-3982325/v1.

Program: Collaborative Research Network
Team:
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Immunofluorescence Staining in Mouse Brain Tissue Sections

Protocol outlines immunofluorescence staining in mouse brain tissue sections.

Program: Collaborative Research Network
Team:
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Western Blot in Mouse Brain Tissue for detecting pRab proteins

Protocol for detecting pRab proteins in mouse brain tissue.

Program: Collaborative Research Network
Team:
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ImmPRESS Polymer Detection Following BaseScope In Situ Hybridization

Perform ImmPRESS immunostaining with alkaline phosphatase labeling on BaseScope In Situ Hybridization mouse brain sections following standard protocol with modifications for optimal results.

Program: Collaborative Research Network
Team:
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3,3-Diaminobenzidine Tetrahydrochloride (DAB) Immunohistochemistry on Mouse Brain Tissue Sections

This protocol details the staining for free floating mouse brain sections.

Program: Collaborative Research Network
Team:
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Ventral Midbrain Genomic PCR

Protocol outlines ventral midbrain genomic PCR for research purposes.

Program: Collaborative Research Network
Team:
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