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Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Feeder-free culturing of hPSCs
This collection describes the standard procedure of feeder-free culturing of human pluripotent stem cells (hPSCs) using mTeSR-plus or StemFlex
Coating plates
This protocol describes the process of coating plates using either VTN, Matrigel or Geltrex for use in culturing of feeder-free human pluripotent stem cells (hPSCs)
Subcloning of genotype-confirmed hPSCs clones
This protocol describes a standard procedure for subcloning of genotype-confirmed human pluripotent stem cells (hPSCs).
Thawing, Passaging and Freezing of hPSCs on MEFs
This collection contains protocols which describe the standard procedure of culturing human pluripotent stem cells (hPSCs) on inactivated mouse embryonic fibroblasts (MEFs).
Preparing mRNA for nucleofection of hPSCs
This protocol describes the standard procedure for preparing mRNA to be delivered into human pluripotent stem cells (hPSCs) using nucleofection.
Sample preparation for aCGH karyotyping
This protocol describes the standard procedure preparing cell pellets from human pluripotent stem cells (hPSCs) cultured on MEFs for outsourced aCGH karyotyping.
Single cell survival assay
This protocol describes the experimental procedure used to measure single cell survival rates post nucleofection of human pluripotent stem cells (hPSCs). General Notes: 1. Throughout these protocols, the term hPSC is used to collectively refer to…
Culture and transfection of HEK293T cells
This protocol describes a standard procedure culturing and transfecting HEK293T cells Protocol overview: A. Culturing HEK293T cells B. Transfection of HEK293T cells with Lipofectamine 2000
Px330-EGFP-LRRK2-CRISPR/Cas9
It can be used to introduce LRRK2-G2019S mutation using CRISPR/Cas9 based genome editing.
Highly efficient generation of isogenic pluripotent stem cell models using prime editing
Prime editing (PE) simplifies creating human pluripotent stem cell (hPSC) disease models by optimizing mRNA delivery with editing efficiency increased up to 13-fold, enabling correction or introduction of Parkinson's disease mutations in hPSCs.
Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs
This collection describes the standard procedure for the delivery of plasmids, mRNA or ribonucleoprotein (RNP) into human pluripotent stem cells (hPSCs) using nucleofection or electroporation.
Electroporation of hPSCs
This protocol describes the standard procedure for the delivery of plasmids into human pluripotent stem cells (hPSCs) using electroporation.
FACS-based enrichment of transfected hPSCs
This protocol describes the procedure FACS-based enrichment of transfected human pluripotent stem cells (hPSCs).
Seeding nucleofected hPSCs in 96-well plates using limited dilution
This protocol describes a standard procedure for seeding nucleofected human pluripotent stem cells in 96-well plates using limited dilution. This protocol follows nucleofection of hPSCs as described in detail in the collection "Nucleofection…
Highly efficient generation of isogenic pluripotent stem cell models using prime editing – Datasets
This collection contains the following datasets related to the paper Highly efficient generation of isogenic pluripotent stem cell models using prime editing 1. AAVS1 knock-in genotyping 2. aCGH karyotyping 3. Tabular datasets for associated…