Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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ATP13A4 gates extracellular polyamine levels to control excitatory synaptogenesis
Polyamines are crucial for brain function. ATP13A4 is the main polyamine transporter in astrocytes, impacting astrocyte morphology, synapse formation, and neurodevelopment. Mutations in ATP13A4 are linked to neurodevelopmental disorders.
Raw datasets for “Cholesterol Dysregulation in APOE4 Astrocytes Promotes α-Synuclein Pathology in miBrains, a Human Brain Tissue Model”
This dataset contains all primary data and quantifications
Methods for “Cholesterol Dysregulation in APOE4 Astrocytes Promotes α-Synuclein Pathology in miBrains, a Human Brain Tissue Model”
Collection of protocols used for the manuscript entitled "Cholesterol Dysregulation in APOE4 Astrocytes Promotes α-Synuclein Pathology in miBrains, a Human Brain Tissue Model".
A druggable ATP13A3–antizyme switch controls adaptive polyamine uptake in cancer
Cellular polyamine depletion for cancer treatment is hindered by compensatory uptake. ATP13A3, not ATP13A2, is the key polyamine importer affected by DFMO. Antizyme inhibits uptake by ATP13A3, suggesting a targetable strategy for cancer therapy.
Culturing, transfection and NBD-labeled lipid uptake assay in HeLa-CDC50A-OE cells
Assay for NBD-labeled lipid uptake in HeLa-CDC50A-OE cells includes seeding, transfection, and flow cytometry to measure flippase construct activity, enabling quantitative assessment of lipid uptake.
Flow cytometry data from the manuscript “Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mamma-lian Cells”
Summary: The manuscript presents a fluorescence-gated flow cytometry method to measure lipid flippase activity in mammalian cells.
Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency
Pathogenic variants in ATP13A2 cause Kufor-Rakeb syndrome & early-onset parkinsonism. Spermidine supplementation rescues symptoms by counteracting microglia dysfunction & improving neuronal integrity.
all protocols related to “Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency”
All protocols related to "Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency"
Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mammalian Cells
P4-ATPase lipid flippases create lipid asymmetry in cells. A new strategy using NBD-lipid uptake assays improves sensitivity and analysis of ATP11C function. This method enhances studying regulatory interactions in mammalian cells.
pCHMWS-hATP13A4_A356V-IRES-Puro
transfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant
pCHMWS-hATP13A4_A356V-Twinstrep-Flag-IRES-Puro
transfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant with Twinstrep-Flag tag
AAV-gfaABC1D-Lamp1-TurboID-HA
Using the gfaABC1D promoter expressing Lamp1-TurboID fusion protein
pCHMWS-FLUC-IRES-Puro
transfer plasmid for lentiviral vector production expressing firefly luciferase (FLUC)
Generation of stable cell lines (Lentiviral vectors)
Generation of stable cell lines.
AAV-gfaABC1D-cytoTurboID-HA
Using the gfaABC1D promoter expressing cytoplasmic TurboID with C-terminal HA tag
