Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
all protocols related to “Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency”
All protocols related to "Spermidine suppresses glial inflammation and parkinsonian abnormalities in ATP13A2 deficiency"
Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mammalian Cells
P4-ATPase lipid flippases create lipid asymmetry in cells. A new strategy using NBD-lipid uptake assays improves sensitivity and analysis of ATP11C function. This method enhances studying regulatory interactions in mammalian cells.
pCHMWS-hATP13A4_A356V-IRES-Puro
transfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant
pCHMWS-hATP13A4_A356V-Twinstrep-Flag-IRES-Puro
transfer plasmid for lentiviral vector production expressing Hs ATP13A4 A356V mutant with Twinstrep-Flag tag
AAV-gfaABC1D-Lamp1-TurboID-HA
Using the gfaABC1D promoter expressing Lamp1-TurboID fusion protein
pCHMWS-FLUC-IRES-Puro
transfer plasmid for lentiviral vector production expressing firefly luciferase (FLUC)
Generation of stable cell lines (Lentiviral vectors)
Generation of stable cell lines.
AAV-gfaABC1D-cytoTurboID-HA
Using the gfaABC1D promoter expressing cytoplasmic TurboID with C-terminal HA tag
Postnatal astrocyte labeling by electroporation
Here, we provide a step-by-step guide to perform Postnatal Astrocyte Labeling by Electroporation (PALE), detailing the preparation, electroporation procedure, and post-electroporation analysis.
in vivo TurboID
Protocol uses iBioID with Lamp1-TurboID in astrocytes to study endo-/lysosomal protein interactions in mice. AAV delivery allows selective biotinylation for proteome mapping, offering insights into astrocyte signaling in vivo.
Immunocytochemistry – subcellular localization of ATP13A4
This protocol describes an optimized method for visualizing the subcellular localization of over-expressed ATP13A4 in HeLa cells using immunocytochemistry.
IHC staining (DAB) of Iba1 and Gfap
This protocol details the IHC staining (DAB) of Iba1 and Gfap.
Protein quantification by SDS-PAGE and Coomassie staining
This protocol provides a step-by-step guide for quantifying protein concentration by SDS-PAGE and Coomassie staining.
Immunoblotting protocol
This protocol outlines a step-by-step method for performing western blot analysis, including protein separation by gel electrophoresis, transfer to membranes, antibody incubation, and detection.
Expression and purification of ATP13A4 from Saccharomyces cerevisiae
This protocol describes the expression and purification of human ATP13A4 with a C-terminal Twin-Strep tag from Saccharomyces cerevisiae using a CuSO₄-inducible system.
