Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Flow cytometry data related to ‘The lipid flippase ATP10B enables cellular lipid uptake under stress conditions’
Flow cytometry data related to 'The lipid flippase ATP10B enables cellular lipid uptake under stress conditions'
Lipidomic analysis in HeLa cells for HexCer levels
A protocol for the lipidomics analysis in HeLa cells for HexCer levels is described.
Lipidomic analysis in HeLa cells
A protocol for the lipidomic analysis in HeLa cells is described.
Lipidomic analysis in WM115 cells
A protocol for the lipidomic analysis in WM115 cells
Midbrain organoid differentiation in spinner flasks
Midbrain differentiation protocol using spinner flasks
ATP13A2-mediated endo-lysosomal polyamine export counters mitochondrial oxidative stress
Recessive loss-of-function mutations in ATP13A2 (PARK9) are associated with a spectrum of neurodegenerative disorders, including Parkinson’s disease (PD). We recently revealed that the late endo-lysosomal transporter ATP13A2 pumps polyamines like…
pLenti HsATP10B_WT-T2A-His-flag-TMEM30A
Transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A.
Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disorders
ATP13A2 is a late endolysosomal transporter that exports the polyamines spermine and spermidine from the organellar lumen to the cytosol. Loss-of-function variants in ATP13A2 are causative for Kufor-Rakeb syndrome (KRS, a recessive juvenile-onset…
P5B-ATPases in the mammalian polyamine transport system and their role in disease
Polyamines (PAs) are physiologically relevant molecules that are ubiquitous in all organisms. The vitality of PAs to the healthy functioning of a cell is due to their polycationic nature causing them to interact with a vast plethora of cellular…
pLenti HsATP13A3 WT
Transfer plasmid for lenti viral vector production, expresses wild-type Homo sapiens ATP13A3
Radiolabeled polyamine uptake in cells
This protocol provides a technique to determine the radiolabeled polyamine uptake capacity in cells, via the acquisition of disintegrations per minute (DPM) using a Liquid Scintillation Counter.
Fluorescently labeled polyamine uptake (via Flow Cytometry)
Assess polyamine uptake capacities of a specific cell line after incubation with fluorescently labeled polyamines and mean fluorescence intensity acquisition via flow cytometry.
Western blotting to detect ATP13A2 and ATP13A3
Protocol to detect ATP13A2 and ATP13A3 via Western Blotting.
The lipid flippase ATP10B enables cellular lipid uptake under stress conditions
ATP10B mutations are linked to Parkinson's and Lewy body disease. ATP10B acts as a lipid transporter in late endo-/lysosomes, enhancing phosphatidylcholine uptake in cells under stress conditions like rotenone treatment.
Microsomal membrane isolation from cell culture
Microsomal membrane isolation from cell culture
