Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Multi-omics reveal critical differentiation target for Parkinson’s Disease-vulnerable midbrain dopaminergic neurons
We describe a multiomic-guided strategy that enriches SOX6+ mDA neurons by combining enhancing Sonic Hedgehog agonism with prolonged Wnt activation. This work is a reproducible differentiation platform for generating the PD-susceptible mDA subtype
Extracellular space diffusion modelling identifies distinct functional advantages of archetypical glutamatergic and GABAergic synapse geometries
We modelled extracellular diffusion in super-resolved images of live brain tissue neuropil. We found that extracellular space geometry shapes local diffusion and this has functional implications for signaling arising from synaptic spill-over.
Beam test
Beam test for mice
IBA1 Immunochemistry on human paraffin sections
Iba1-specific Immunochemistry protocol on paraffin-embedded human brain sections
Olympus Slideview VS200″ slide scanning
Brief guide on the main steps to follow in order to scann a sample with the "Olympus Slideview VS200 slide scanner. Although examples for certain parameters are presented, each user should choose them based on the characteristics of their samples.
Flow Cytometry
Flow Cytometry
Cytokine Array
The EMD MILLIPLEX‱ MAP Human Cytokine/Chemokine/Growth Factor Pane A – Immunology Multiplex Assay (Merck Millipore) is employed to simultaneously analyse TNF-α, IL-1β, IL 6, and INFγ with Bead-Based Multiplex Assay using the Luminex‱…
2D and 3D Neuron/Microglia co-culture systems
2D and 3D Neuron/Microglia co-culture systems
Gene expression analysis
To evaluate whether human microglia cells (hMG) can be activated by neuromelanin (NM), hMG were cultured with and without the presence of NM for 24 h and then harvested to measure gene expression of selected pro-inflammatory and neurotoxic factors.
Immunocytochemistry (ICC) detection of key microglia markers in iPSC derived human microglia cells
Immunocytochemistry (ICC) detection of key microglia markers in iPSCderived human microglia cells (hMG)
Intracellular ROS measurement in iPSC-derived microglia
Intracellular ROS measurement in iPSC-derived microglia
iPSC-derived organoid generation
iPSC-derived organoid generation
iPSC derived microglia (hMG) culture
iPSC derived microglia (hMG) culture
iPSC-derived vmDAn
iPSC-derived vmDAn
Neuromelanin phagocytosis assay
Neuromelanin phagocytosis assay
