Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Adult-specific Reelin expression alters striatal neuronal organization: implications for neuropsychiatric disorders
Reelin levels might modulate the numbers of striatal interneurons and the density of the nigrostriatal dopaminergic projections, suggesting that these changes may be involved in the protection of Reelin against neuropsychiatric disorders.
Rat brain processing for histological analyses
Protocol for rat brain processing for histological analyses.
Cylinder behavioral test
Protocol for cylinder behavioral test for rats.
Stereotaxic rat brain surgery for Substantia Nigra targeting
Protocol for stereotaxic rat brain surgery for Substantia Nigra targeting.
Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions
The authors developed an iPSC toolbox utilizing piggyBac-based or targeted transgenes to rapidly induce CNS cells with concomitant expression of aggregation-prone proteins.
RNA and protein extraction from bulk dissections
Protocol for RNA and protein extraction from bulk dissections.
cDNA synthesis
Protocol for cDNA synthesis.
ImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections
ImageJ FIJI code to semi-automatize the morphological analysis of microglia in histological sections.
In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease
Using a newly developed rodent model, the authors assessed whether the intracellular buildup of neuromelanin that occurs with age can be slowed down in vivo to prevent or attenuate Parkinson’s disease.
Gene expression analysis by qPCR
Protocol for gene expression analysis by quantitative Real-Time PCR (qPCR).
Stereology-mediated cell counting using StereoInvestigator
Protocol for stereologic count with StereoInvestigator.
Immunofluorescence on paraffin sections
Protocol for immunofluorescence on paraffin sections.
Intracellular neuromelanin quantification
Protocol for intracellular neuromelanin quantification.
Neuromelanin accumulation drives endogenous synucleinopathy in non-human primates
A new NHP PD model was developed by inducing neuromelanin (NM) accumulation in dopaminergic neurons, leading to synucleinopathy and neuron degeneration similar to human PD neuropathology. Lowering NM levels may offer PD therapeutic strategies.
Immunochemistry on paraffin sections
Protocol for immunochemistry on paraffin sections.
