Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
MANUAL CELL COUNTING
MANUAL CELL COUNTING
FISH CODETECTION
DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
Solutions used for FISH Codetection
Protocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
Astrocytes mediate the dopaminergic modulation of tonic GABAergic signaling in substantia nigra
To characterize the actions of dopamine on substantia nigra pars reticulata (SNr) GABAergic neurons, optogenetic and electrophysiological tools were used in ex vivo mouse brain slices to monitor synaptic transmission arising from neurons.
FISH representative images, tabular datasets, and 3D light-sheet microscopy videos
FISH representative images, tabular datasets, and 3D light-sheet microscopy videos of the STN/pSTN.
Python code: NHP rendering
Annotated Python package for NHP image processing and visualization.
Python code: Lightsheet Alignment
Code used to align a moving light-sheet specimen to a fixed reference through their autofluorescence (AF) channels.
Python code: rendering mouse atlas
This script extracts STN and PSTN from an Allen CCF annotation volume and produces smoothed VTK meshes and an orthographic rotation series.
Python code: rendering NHP atlas
This script reconstructs bilateral rhesus macaque STN and PSTN surfaces from digitized serial Paxinos contours.
Single-cell extracellular recordings (surgery, STN optotagging, LHb recordings)
Protocol for single cell extracellular recordings (surgery, STN optotagging, LHb recordings)
Electrophysiological Recordings
Protocol for electrophysiological patch clamp/voltage clamp recordings.
Ex vivo electrophysiology in STN, EP and VP neurons
Protocol for Ex vivo electrophysiology.
Tabular datasets for “A role for the subthalamic nucleus in aversive learning”
Tabular datasets for "A role for the subthalamic nucleus in aversive learning." Includes FISH, electrophysiological, and behavioral datasets.
Opto-Open Field Test
Protocol for optogenetic open field test, which records mouse behavior in an open field arena in response to alternating 5-minute periods of optogenetic stimulation.
Stereotaxic virus injection and fiber optic probe implantation
This protocol serves to prepare mice for in vivo optogenetic stimulation.
