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Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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FISH CODETECTION
DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections
MANUAL CELL COUNTING
MANUAL CELL COUNTING
Solutions used for FISH Codetection
Protocol to make the solutions used in the protocol published by AWM lab for FISH Codetection
Determining the effects of mecamylamine in the mouse striatum using a Conditioned Preference Place (CPP) paradigm
Protocol tests mecamylamine effects in mouse striatum using CPP paradigm to measure preference for drug-associated environment.
Intracranial injections of viral vectors in mouse midbrain and striatum
Protocol for injecting viral vectors in mouse midbrain and striatum is provided.
Immunocytochemistry of acute brain slices used in ex vivo voltammetry recordings
Protocol labels ChAT for cholinergic interneurons and TH for dopaminergic neurons in mouse brain slices. Immunofluorescence used post ex vivo voltammetry recordings on 300-µm thick slices.
In vivo voltammetry and fiber photometry in the mouse striatum
Protocol measures dopamine and calcium levels in striatal dopaminergic axons in vivo via voltammetry and fiber photometry in response to electrical stimulation.
Wide-field imaging of voltage sensors expressed in ex vivo mouse brain slices
This protocol describes how to perform wide-field imaging of voltage sensors using high frame rates (660 Hz minimum every 2.5 minutes) in mouse midbrain using ex vivo brain slices.
Imaging axonal calcium dynamics in ex vivo mouse brain slices
Protocol for imaging calcium dynamics in striatal dopaminergic axons in ex vivo mouse brain slices using GCaMP6f in DAT-Cre:Ai95D mice. Calcium transients were observed in response to single and train electrical stimuli.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices following electrical or optogenetic stimulations
This protocol is to assess changes in extracellular dopamine concentration following electrical or optogenetic stimulations in ex vivo mouse brain slices.
Datasets and Key Resources Table used in Zang, Y. et al. (2025) “An axonal brake on striatal dopamine output by cholinergic interneurons”
This repository contains a 1) Key Resources Table with details on key resources and the persistent identifiers for protocols and code used and generated in this study; 2) Source Data Folder, and 3) Supplementary Data Folder.
Microinvasive Probes for Monitoring Electrical and Chemical Neural Activity in Nonhuman Primates
We designed carbon fiber sensors that enable long-term simultaneous recording of both neurochemical and electrical neural activity in deep brain structures of nonhuman primates.
Custom MATLAB scripts related to Zhang, Y. et al (2025) “An axonal brake on striatal dopamine output by cholinergic interneurons”
MATLAB scripts written by Yan-Feng Zhang to predict how nicotinic receptors impact on dopamine transient in vivo during the dynamic tonic and multiphasic activity in cholinergic interneurons.
s-µIP Fabrication Process
This protocol describes the fabrication of s-µIP microelectrodes. These devices are made of either one or two parylene-coated tungsten-carbon fiber assemblies, threaded through a silica tube.
Aspiration of Fluid in Semi-Sealed Chamber
Protocol for post-op maintenance of electrode chambers emphasizes fluid aspiration and contamination prevention to reduce infection risk. Regular cleaning is crucial for asepsis and preventing complications like meningitis.