Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Data and code for Active Escape (“Integrated Representations of Threat and Control in the Lateral Frontal Pole”)
Data, Code, and Key Resource Table used in the preprint from Stasiak et al., 2025 ("Integrated Representations of Threat and Control in the Lateral Frontal Pole").
Active Escape Protocol (Stasiak et al., 2026)
Protocol for Active Escape task by Stasiak et al., referenced in "Integrated Representations of Threat and Control in the Lateral Frontal Pole" (2026) manuscript.
AAV-gfaABC1D-Lamp1-TurboID-HA
Using the gfaABC1D promoter expressing Lamp1-TurboID fusion protein
AAV-gfaABC1D-cytoTurboID-HA
Using the gfaABC1D promoter expressing cytoplasmic TurboID with C-terminal HA tag
Postnatal astrocyte labeling by electroporation
Here, we provide a step-by-step guide to perform Postnatal Astrocyte Labeling by Electroporation (PALE), detailing the preparation, electroporation procedure, and post-electroporation analysis.
in vivo TurboID
Protocol uses iBioID with Lamp1-TurboID in astrocytes to study endo-/lysosomal protein interactions in mice. AAV delivery allows selective biotinylation for proteome mapping, offering insights into astrocyte signaling in vivo.
RNA isolation and cDNA synthesis
This protocol details the isolation of RNA, purification, and subsequent cDNA synthesis for downstream applications such as qPCR.
RT-qPCR (primary cells)
Protocol details qPCR method for Atp13a4 expression in astrocytes and neurons using Fast SYBR Green Master Mix in a 96-well format. Normalization to 18S gene ensures accurate quantification for reliable gene expression analysis.
Lentiviral vector production and primary astrocyte transduction
Protocol for lentivirus production & transduction into astrocytes is outlined, allowing efficient transduction & stable selection. Facilitates research on primary gene function in astrocytes.
Astrocyte morphology assessment in astrocyte-neuron co-cultures
This protocol outlines the process for analyzing astrocyte morphology in astrocyte-neuron co-cultures using immunostaining and Sholl analysis.
Multiplexed immunofluorescence and RNA-FISH
Protocol detects Atp13a4 mRNA in astrocytes of visual cortex using immunofluorescence and RNA-FISH. Sections from Aldh1L1-EGFP mice are stained, hybridized with probes, and imaged for spatial analysis of Atp13a4 expression in postnatal development.
Glia-Free Cortical Neuronal Feeding Schedule – Neuronal Morphology
This protocol outlines the treatment of cortical neurons with astrocyte-conditioned medium (ACM) or spermidine and subsequent analysis of dendrite length.
Astrocyte Isolation and Transfection for Astrocytes/Neuron co-culture assay
Protocol for isolating rat cortical astrocytes and producing astrocyte conditioned media for synaptogenesis assays.
Synapse staining – mouse brain sections
This protocol details a method to visualize and quantify excitatory synapses using mouse brain sections.
Isolation and culture of primary mouse astrocytes and neurons
This protocol details the methodology to establish primary murine astrocyte and neuron cultures.
