Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Activation of transposable elements is linked to a region- and cell-type-specific interferon response in Parkinson’s disease
Study explores transposable elements role in Parkinson's disease neuroinflammation. TE activation in specific brain regions correlates with innate immune responses, indicating potential involvement in chronic neuroinflammation and PD progression.
T cell purification and activation
This protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen
Release Notes – ASAP CRN Cloud – Version 5.0.0
ASAP CRN Cloud released version 5.0.0 which includes human metagenomics, gastrointestinal spatial transcriptomics, and new PMDBS data (ATAC seq and ParseBio). This release also introduced mouse proteomics, metabolomics, lipidomics, and metagenomics.
Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
This methods paper outlines silencing transposable elements in hiPSCs using CRISPRi. Describes gRNA design, validation via multiome approach. Enables functional studies on TE transcription in hiPSC models.
Adoptive Activated T cell Transfer Protocol
Isolation, in vitro activation, and intraperitoneal delivery of TCR-transgenic CD8+ T cells
Extracellular Markers Staining Protocol for Flow Cytometry
Flow cytometry analysis
Intracellular Cytokine Staining (ICS) Protocol for FACS Analysis
Intracellular staining of lymphocytic cells for Fluorescence-Activated Cell Sorting (FACS) analysis
A single Citrobacter rodentium infection in Pink1 knockout and wild type mice leads to regional blood-brain-barrier perturbation and limited microglial activation without dopamine neuron axon terminal loss
Research suggests immune system activation may contribute to Parkinson’s disease development. Mild infections can increase BBB permeability, triggering brain inflammation in genetically susceptible individuals, potentially leading to PD pathology.
Dataset for “A single Citrobacter rodentium infection in Pink1 knockout and wild-type mice leads to regional blood-brain-barrier perturbation and limited microglial activation without dopamine neuron axon terminal loss”
Immune system activation may contribute to PD development. Infection increases BBB permeability in mice, leading to brain inflammation. This suggests infections could trigger PD in genetically susceptible individuals.
Generation of DATcrePink1 Knockout Mice Expressing tdTomato in Dopaminergic Neurons
Protocol details generation of DATcrePink1 mice expressing tdTomato in dopaminergic neurons. Covers Pink1 KO, HET, WT variants, animal housing, breeding, and genotyping procedures.
Semi-automated quantification of microglial CD68 and Iba1 immunohistochemistry signal in mouse brain slices
A standardized method for semi-automated detection and quantification of CD68 and Iba1 in brain sections using image acquisition, AI segmentation, and data analysis.
Automated Quantification of Dopaminergic Neurons in Floating Sections V.1
Automated method for detecting and measuring dopaminergic neurons in brain sections using image capture, AI segmentation, and data analysis in SNc and VTA regions.
STING dampens the unfolded protein response to enable the presentation of self antigens on MHC-I during inflammation
STING pathway regulates antigen presentation through the UPR, impacting immune response in PD and autoimmune diseases. Targeting STING and UPR could be therapeutic.
Dataset for “STING dampens the unfolded protein response to enable the presentation of self-antigens on MHC-I during inflammation”
STING pathway activation impacts antigen presentation through the UPR and may be a target for therapeutic intervention in PD and autoimmune diseases.
