Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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ASAP CRN Cloud Release Notes – Version 4.0.0
ASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New harmonized collections and individual datasets were added, enhancing research possibilities.
Myeloid PINK1 represses mtDNA release and immune signaling that impacts neuronal pathology in patient-derived idiopathic PD models
PINK1 represses mtDNA release and STING/NF-κB activation in peripheral macrophages of PD model laying the foundation for understanding PINK1-related peripheral mechanisms in idiopathic PD and providing targets for further therapy development
Protocols Suite — SH-SY5Y / iCell® Neurons / High-Content Imaging
Maintenance of SH-SY5Y cells with SNCA overexpression in DMEM/F-12, differentiation with RA/BDNF, and culture of iCell DopaNeurons with SNCA mutation. Treatment and immunostaining for downstream assays using specific protocols and analysis tools.
GCase activity assay
Protocol measures GCase activity using 4-MUG substrate. GCase protein from a cellular model is diluted in acidic buffer to mimic lysosome pH. Adding lipid/detergent maintains enzyme activity. Taurocholate bile salt is included in the protocol.
Immunofluorescencent Staining of A-synuclein, TFEB, GCase and LAMP1 in treated cultured cells
Protocol used to detect a-synuclein, TFEB, GCase, and LAMP1 via immunofluorescence in SH-SY5Y cells with mutant SNCA gene.
Protocol — DQ™ Red BSA Lysosomal Activity Assay HCS Analysis
Lysosomal proteolysis can be measured using self-quenched DQ™ Red BSA, followed by confocal imaging for spot-based quantification.
Protocol — Quantitative Real-Time PCR (qRT-PCR): 3K-SNCA cells
Gene expression in 3K-SNCA SH-SY5Y cells was analyzed using Qiagen RNA extraction and TaqMan qRT-PCR on QuantStudio 1 platform for differentiated and undifferentiated cells.
Protocol — LysoTracker® Red DND-99 Live-Cell Staining 26 HCS Analysis
Text summary: Live-cell lysosomal staining done with LysoTracker Red DND-99, followed by high-content confocal imaging using Opera Phenix Plus, and automated quantification in Harmony/Columbus.
Processing Serum, Plasma, Plasma EDTA from Whole Blood
Protocols cover serum, plasma, and plasma EDTA processing from whole blood samples.
Isolation of Classical Monocytes from PBMCs (MB Kit No. 130-117-337)
Protocol isolates classical monocytes from peripheral blood mononuclear cells.
Stool Sample Collection Protocol
The protocol outlines procedures for stool sample collection.
Isolation of Platelets Protocol
The text describes a platelet isolation protocol.
PBMCs isolation with CT Tubes (8mL Whole blood)
Protocol isolates peripheral blood mononuclear cells using CPT tubes.
Metagenomics sequencing data of publication doi.org/10.3389/fnins.2025.1546203
The study explores the connection between *GBA1* gene variants and gut microbiome in a mouse model, shedding light on Parkinson's disease development.
MSP abundance table associated with doi.org/10.3389/fnins.2025.1546203
Summary: The table displays the abundance of microbial species in the gut of GBA1 L444P/WT mouse model, exploring the connection between GBA1 host genotype and gut microbiome in Parkinson's disease research.
