Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
ASAP CRN Cloud Release Notes – Version 4.0.0
ASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New harmonized collections and individual datasets were added, enhancing research possibilities.
western blot data of ‘A new selection strategy to enrich dopaminergic neurons facilitates comparisons between patient iPSC-derived lines’
western blot data of 'A new selection strategy to enrich dopaminergic neurons facilitates comparisons between patient iPSC-derived lines'
Jess western blot automated system
Brief description of preparation of samples for Jess western blot automated system. Detailed instructions are included with the reagent pack
HT707
Induced pluripotent stem cell
HT708
Induced pluripotent stem cell
HT711
Induced pluripotent stem cell
HT707 TH-neo
Induced pluripotent stem cell
HT708 TH-neo
Induced pluripotent stem cell
HT711 TH-neo
Induced pluripotent stem cell
An ImageJ/FIJI Preprocessing Workflow for Multi-Series Confocal Microscopy Datasets Prior To CellProfiler Analysis
A workflow using ImageJ/FIJI aims to create three-slice MIPs from multi-series .czi files to capture weak signals. It involves extracting datasets, creating projections, and saving images for efficient analysis in CellProfiler.
A CellProfiler Pipeline for Quantification of p-SNCA in Mouse Striatal Cholinergic and Medium Spiny Neurons
Brain sections stained for pSNCA, DARPP-32, ChAT, and DAPI. Z-stacks processed into MIPs for analysis. Outputs include pSNCA intensity and neuronal mask area, normalised for cell type-specific burden using R script.
Matters arising: Forced Symmetry Artifacts in a Prohibitin Complex Structure
Lange et al. proposed a model for mitochondrial prohibitin complex with unequal subunit stoichiometry. Data suggests an asymmetric structure, questioning the validity of the proposed model. Care is needed in symmetry imposition in cryo-ET.
pHTN-Halo-TEV-ATG3_C264S
Plasmid for Mammalian Expression of human ATG3 C264S.
pHTN-Halo-TEV-ATG3_H266Q
Plasmid for Mammalian Expression of human ATG3 H266Q.
pHTN-Halo-TEV-ATG3_C264A
Plasmid for Mammalian Expression of human ATG3 C264A.
