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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Dataset
Primary data for article, “Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input” (DOI: 10.1016/j.celrep.2024.114483)
Primary data for the published manuscript, “Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input” (Ryan et al. Cell Reports 43:8, 114483, 2024.).
Article
Excessive firing of dyskinesia-associated striatal direct pathway neurons is gated by dopamine and excitatory synaptic input
The authors explored the cellular and synaptic mechanisms of levodopa-induced dyskinesia (LID), a complication of Parkinson’s disease therapy characterized by involuntary movements. Findings suggest how the intrinsic and synaptic properties of heterogeneous dMSN subpopulations integrate to support action selection.
Article
Tonic dendritic GABA release by substantia nigra dopaminergic neurons
Preprint: Recent studies have demonstrated the importance of extrastriatal dopamine release in motor deficits of Parkinson’s disease (PD). To characterize the actions of dopamine on substantia nigra pars reticulata (SNr) GABAergic neurons, optogenetic and electrophysiological tools were used in ex vivo mouse brain slices to monitor synaptic transmission arising from neurons.
Code
Fiber photometry data analysis
Custom code for fiber photometry data pre-processing (bleaching correction, DF/F calculation, behavioral parsing…) are provided.
Teams
Protocol
Rotarod Test to assess motor coordination in a mouse parkinsonian model
This protocol evaluates motor coordination in the rotarod assay in parkinsonian mice.
Teams
Protocol
Acute striatal or midbrain fiber photometry in head-fixed mice
While this protocol focuses recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.
Teams
Protocol
Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
This behavior is used to assess fine motor movements in a mouse Parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
Teams
Protocol
Catalepsy test (Bar test)
The catalepsy test (bar test) was developed to test motor coordination and motor impairments.
Teams
Protocol
Create mask for axonal quantification analysis with FIJI
This protocol describes how to create a quantified mask from an image of fluorescence-tagged axonal projections using FIJI/ImageJ software.
Teams
Protocol
Endosome isolation
Subcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
Teams
Protocol
Genotyping mice from ear clips
This protocol describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjusted.
Teams
Protocol
Immunofluorescence staining for postmortem mouse brain tissue
This is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
Teams
Teams
Protocol
Isolation and processing of embryonic and postnatal brains
This protocol describes:
-Harvesting embryos from females from embryonic day 10 [e10] to embryonic day 18 [e18]
-Brain isolation of embryonic (e10-e18) and postnatal brains (p0-p30)
-Processing and freezing of embryonic and postnatal brains.
Teams