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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Protocol
Isolation and processing of embryonic and postnatal brains
This protocol describes:
-Harvesting embryos from females from embryonic day 10 [e10] to embryonic day 18 [e18]
-Brain isolation of embryonic (e10-e18) and postnatal brains (p0-p30)
-Processing and freezing of embryonic and postnatal brains.
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Protocol
Immunofluorescence staining for postmortem mouse brain tissue
This is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
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Protocol
Immunofluorescent labeling of cultured cells
This protocol describes the process of Paraformaldehyde fixation [PFA-fixation] and immunofluorescent-labeling of cultured cells.
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Protocol
Coating coverslips for cell culture
Coated coverslips provide a nourishing adherent surface for cell culture. This protocol provides step by step instruction on how to coat coverslips for cell culture.
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Article
R1441C and G2019S LRRK2 knock-in mice have distinct striatal molecular, physiological, and behavioral alterations
Publication: LRRK2 mutations are closely associated with PD. Convergent evidence suggests that LRRK2 regulates striatal function. Here, by using knock-in mouse lines expressing the two most common LRRK2 pathogenic mutations – G2019S and R1441C – the authors investigated how LRRK2 mutations altered striatal physiology. View original preprint.
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Article
Dopaminergic axons track somatic signaling in behaving mice
Preprint: Striatal dopamine released from the axons of midbrain dopamine neurons has been linked to a wide range of functions, including movement control and reward-based learning. Here, authors used genetic strategies to isolate key dopaminergic neuron subtypes and monitor their axonal and somatic signaling patterns in behaving mice.
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Article
Molecular heterogeneity in the substantia nigra: a roadmap for understanding PD motor pathophysiology
Review: This article discusses the existing knowledge of DA neuron subtypes and attempts to provide a roadmap for how their distinctive properties can provide novel insights into the motor symptoms of Parkinson’s disease (PD).
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Protocol
Acute striatal or midbrain fiber photometry in head-fixed mice
While this protocol focuses recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.
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Protocol
Adhesive Removal Test to assess sensorimotor deficits in parkinsonian mice
This behavior is used to assess fine motor movements in a mouse Parkinsonian model. It checks for correct paw and mouth sensitivity (time-to-contact) and correct dexterity (time-to-remove).
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Protocol
Catalepsy test (Bar test)
The catalepsy test (bar test) was developed to test motor coordination and motor impairments.
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Protocol
Create mask for axonal quantification analysis with FIJI
This protocol describes how to create a quantified mask from an image of fluorescence-tagged axonal projections using FIJI/ImageJ software.
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Protocol
Genotyping mice from ear clips
This protocol describes the genotyping procedure from ear clip samples. This includes a general PCR protocol for primers with annealing temperatures in the range 55-70 degrees C. For other primers, the thermal cycling should be adjusted.
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Protocol
Endosome isolation
Subcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
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