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Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
Alpha-synuclein overexpression can drive microbiome dysbiosis in mice
Growing evidence indicates that persons living with Parkinson’s disease (PD) have a unique composition of indigenous gut microbes. Here, the authors used a transgenic mouse strain, which overexpress wild-type human α-syn to test how the gut microbiome composition responds in this model of PD pathology during aging.
3′ mRNA-sequencing
Protocol for 3′ mRNA sequencing of mouse gastrointestinal tract tissues.
Datasets associated with “Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology”
This RNA data set corresponds to the preprint: Neuronal Activation of the Gastrointestinal Tract Shapes the Gut Environment in Mice (Yoo et al., 2021).
Dopamine transporter and synaptic vesicle sorting defects initiate auxilin-linked Parkinson’s disease
Published: Auxilin helps in recycling of synaptic vesicles to facilitate neurotransmission and loss of auxilin is associated with PD. The authors show auxilin knockout mice exhibit typical PD pathology, dopamine transport is disrupted due to slower dopamine reuptake kinetics, and that macroautophagy and defective synaptic vesicle sorting contributes to dopamine dyshomeostasis. View original preprint.
Peripheral neuronal activation shapes the microbiome and alters gut physiology | bioRxiv
Preprint: The enteric nervous system shapes the intestinal environment and communicates with various brain organs, including the brain. The authors used recombinant adeno-associated viral (rAAV) vectors and chemogenetics to map and activate enteric neurons in mice with spatial and temporal resolution.
Neural activation of the GI tract
Protocol for neuronal activation of the enteric nervous system.
Virus production and administration
Protocol for AAV production and administration to mice.
Tissue preparation, immunohistochemistry, imaging, and quantification
Protocol for neuronal imaging.
GCaMP6f fluorescence in ex vivo intestinal preparation
Protocol for GCaMP6f imaging in ex vivo mouse intestinal tissue.
Metagenome preparation and analysis
Protocol for metagenomic analysis from mouse fecal pellets.
Metabolomics preparation and analysis
Protocol for metabolomic analysis from mouse fecal and cecal samples.
Proteome preparation and analysis
Protocol for proteomic analysis from mouse cecal samples.
Integrated multi-cohort analysis of the Parkinson’s disease gut metagenome
Preprint: Here, the fecal metagenomes of those living with PD compared to others in the household were profiled from 4 geographically-distinct sites across 3 continents. The question was whether there were any specific PD-associated signatures in gut microbiome that are either enriched or depleted in PD.
Whole gut transit time, fecal water content, and fecal output
Protocol for mouse gastrointestinal function assays (whole gut transit time, fecal water content, and fecal output).