Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Article

Dopamine transporter and synaptic vesicle sorting defects initiate auxilin-linked Parkinson’s disease

Preprint: Auxilin helps in recycling of synaptic vesicles to facilitate neurotransmission and loss of auxilin is associated with PD. The authors show auxilin knockout mice exhibit typical PD pathology, dopamine transport is disrupted due to slower dopamine reuptake kinetics, and that macroautophagy and defective synaptic vesicle sorting contributes to dopamine dyshomeostasis.

Article

Integrated multi-cohort analysis of the Parkinson’s disease gut metagenome

Preprint: Here, the fecal metagenomes of those living with PD compared to others in the household were profiled from 4 geographically-distinct sites across 3 continents. The question was whether there were any specific PD-associated signatures in gut microbiome that are either enriched or depleted in PD.

Article

Subcellular proteomics of dopamine neurons in the mouse brain reveals axonal enrichment of proteins encoded by Parkinson’s disease-linked genes

Published: Striatal dopamine axons are thin, highly branched structures that have been difficult to study at the proteomic level. Combining APEX2 biotinylating with mass spectrometry to map the proteomes of midbrain dopaminergic neurons, an enrichment of proteins encoded by PD-associated genes in striatal dopaminergic axons was found. View original preprint.

Article

Unaltered T cell responses to common antigens in individuals with Parkinson’s disease

Preprint: T cells have been shown to be overactive in individuals with PD. The authors tested a wide variety of commonly encountered immune targets on PD and non-PD control derived T cells and observed no differences between their immune responses.

Protocol

Activation Induced Marker (AIM) Staining Protocol

This protocol details about activation induced marker staining.

Article

Subcellular and regional localization of mRNA translation in midbrain dopamine neurons

Published: Midbrain dopaminergic neurons have broad dendritic and axonal arborizations, but local protein synthesis is not understood here. Using, highly sensitive ribosome-bound RNA sequencing and imaging to characterize the translatome, the authors uncovered local mRNA translation of dopamine synthesis, release, and reuptake machinery in dendrites, but not axons. View original preprint.

Article

Subcellular proteomics of dopamine neurons in the mouse brain

Published: Understanding the proteome of dopamine neuron is difficult due to the complex cytoarchitecture of the neurons. The authors were able to map the somatodendritic and axonal proteomes of midbrain dopaminergic neurons. Interestingly, the authors found striatal dopaminergic neurons house most proteins. View original preprint.

Protocol

Intracellular Cytokine (ICS) Staining Protocol

This protocol details about intracellular cytokine (ICS) staining.

Article

Central and Peripheral Inflammation: Connecting the Immune Responses of Parkinson’s Disease

Review: Authors highlight the important work being done that implicates central and peripheral inflammation in playing a role in PD. They also discuss how these two distant inflammations appear related and how that may be mediated by autoantigenic responses to α-synuclein.

Article

Similarities and differences between nigral and enteric dopaminergic neurons unravel distinctive involvement in Parkinson’s disease

Review: In this comparative review, authors compare the Dopamine neurons in the enteric nervous system, substantia nigra pars compacta, and ventral tegemental area, to assess whether, independent of their location or function, it is sufficient for neurons to be dopaminergic to be selectively vulnerable during the progression of PD.

Article

Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures

Published: Recent findings identified PD-associated autoimmune features. Using RNA sequencing, the authors found a broad gene expression profile in memory T cells and a specific PD-associated gene signature. Slpha-synuclein responding T cell gene expression profiles were associated with dysregulation in multiple cellular pathways related to PD genes. View original preprint.