Catalog

ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.

Article

Dopamine transporter and synaptic vesicle sorting defects initiate auxilin-linked Parkinson’s disease

Preprint: Auxilin helps in recycling of synaptic vesicles to facilitate neurotransmission and loss of auxilin is associated with PD. The authors show auxilin knockout mice exhibit typical PD pathology, dopamine transport is disrupted due to slower dopamine reuptake kinetics, and that macroautophagy and defective synaptic vesicle sorting contributes to dopamine dyshomeostasis.

Article

Integrated multi-cohort analysis of the Parkinson’s disease gut metagenome

Preprint: Here, the fecal metagenomes of those living with PD compared to others in the household were profiled from 4 geographically-distinct sites across 3 continents. The question was whether there were any specific PD-associated signatures in gut microbiome that are either enriched or depleted in PD.

Article

Targeted neuronal activation of the gastrointestinal tract shapes the environment of the gut in mice

The enteric nervous system (ENS) integrates cues from the brain and from local signals in the gut to coordinate responses that shape the intestinal milieu. Here, the authors examine the role of neurons in modulating intestinal physiology, mucosal immunity, and gut microbiome structure.

Dataset

Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures – Gene Validation FCS

FCS files corresponding to the gene validation experiment described in “Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures.”

Dataset

Axonal and somatodendritic proteomes of dopamine neurons in the mouse brain

The elaborate cytoarchitecture of DA neurons is embedded within complex brain tissues, making it difficult to access the DA neuronal proteome using conventional methods. This mass spectrometry proteomics dataset is a part of “Subcellular proteomics of dopamine neurons in the mouse brain” (Hobson et al., 2022).

 

Dataset

Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures – Cell Surface FCS

FCS files corresponding to the cell surface experiment described in “Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures.”

Article

Similarities and differences between nigral and enteric dopaminergic neurons unravel distinctive involvement in Parkinson’s disease

Review: In this comparative review, authors compare the Dopamine neurons in the enteric nervous system, substantia nigra pars compacta, and ventral tegemental area, to assess whether, independent of their location or function, it is sufficient for neurons to be dopaminergic to be selectively vulnerable during the progression of PD.

Dataset

Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures – Fluorospot SFC

Fluorospot SFC (combined IFNg, IL-5, and IL-10) data and corresponding HC-PD group IDs described in “Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures.”

Dataset

ENS quantification

Labelled enteric neurons in living mice with recombinant adeno-associated viruses (rAAVs) expressing fluorescent proteins, and used tissue clearing techniques to enhance visualization of intact GI tissue.

 

Dataset

Subcellular and regional localization of mRNA translation in midbrain dopamine neurons

This dataset is a part of “Subcellular and regional localization of mRNA translation in midbrain dopamine neurons” (Hobson et al., 2022) where the authors investigated mRNA localization in midbrain dopaminergic (mDA) neurons, cells with enormous axonal and dendritic projections, both of which can release dopamine (DA).

Dataset

A single-cell atlas of cell types, states, and other transcriptional patterns from nine regions of the adult mouse brain

We report 690K single-cell transcriptomes from nine different brain regions from adult mice (Frontal and Posterior Cortex, Hippocampus, Thalamus, Cerebellum, Striatum, Globus Pallidus externus/Nucleus Basalis, Entopeduncular / Subthalamic Nuclei, & Substantia Nigra / Ventral Tegmental Area).

 

Dataset

Peripheral neuronal activation of the intestinal tract shapes the microbiome and alters gut physiology-RNAseq

This RNA data set corresponds to the preprint: Neuronal Activation of the Gastrointestinal Tract Shapes the Gut Environment in Mice (Yoo et al., 2021).

Code

Subcellular and regional localization of mRNA translation in midbrain dopamine neurons – DropSeqPipeline8

This is a data processing pipeline for analyzing microwell- or DropSeq-like scRNA-seq data. It can also be used for analyzing pooled plate-based scRNA-seq.

 

Article

Central and Peripheral Inflammation: Connecting the Immune Responses of Parkinson’s Disease

Review: Authors highlight the important work being done that implicates central and peripheral inflammation in playing a role in PD. They also discuss how these two distant inflammations appear related and how that may be mediated by autoantigenic responses to α-synuclein.