Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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CRN Team Name
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Collection of protocols of Team Deleidi used in the publication: “”LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease””
Collection of protocols of Team Deleidi used in the publication: ""LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease""
Available ASAP-related hPSC collection from Team Studer
Collection of human pluripotent stem cell lines consisting of isogenic GBA, LRRK2, SNCA series, KI-reporter lines for TOMM20, b-actin, LAMB1, LAMP1, a-synuclein overexpression lines, and other hPSC resources.
Team Science Approaches to Unravel Monogenic Parkinson’s Disease on a Global Scale
In this article, we describe combining both efforts in a merger project resulting in a global monogenic PD cohort with the buildup of a sustainable infrastructure to identify the multi-ancestry spectrum of monogenic PD and enable studies of factors…
SDS and Clear Native Gel Electrophoresis
Protocol outlines SDS-PAGE & clear native PAGE for analyzing α-synuclein, Gba, & related proteins from mouse brain samples.
Adaptor protein-3 produces synaptic vesicles that release phasic dopamine
The burst firing of midbrain dopamine neurons releases a phasic dopamine signal that mediates reinforcement learning. At many synapses, however, high firing rates deplete synaptic vesicles (SVs), resulting in synaptic depression that limits release.…
Postnatal primary hippocampal neuronal cultures
This protocol describes the preparation of postnatal primary hippocampal neuronal cultures.
Mouse Behavior – Open Field and T-Maze
This protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
Live Imaging of Primary Mouse Neuron Cultures
This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging described involves labeling of dopamine neurons with a…
Mouse Brain Tissue Collection and Analysis
This protocol describes the dissection and collection of coronal sections of the striatum and midbrain from a mouse brain. The tissue can be used in a number of applications and here we describe two: the measurement catecholamine levels using…
Immunostaining Mouse Brain Tissue or Neuronal Cultures
This protocol describes immunohistochemical and immunoctyochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for…
The role of α-synuclein in exocytosis
Review focuses on α-synuclein's role in exocytosis, a key process in Parkinson's disease. Despite unclear pathogenesis, evidence points to αsyn's involvement in regulating cellular processes.
Data for Jain et al Adaptor Protein-3 Produces Synaptic Vesicles that Release Phasic Dopamine
Primary data for Jain et al "Adaptor protein-3 produces synaptic vesicles that release phasic dopamine" (Proc Natl Acad Sci. 2023; 120(42):e2309843120. doi: 10.1073/pnas.2309843120)
α-Synuclein binding assay to isolated synaptic vesicles
Binding of α-synuclein to synaptic vesicles was tested using His-tagged α-syn and Co Dynabeads. Mouse brain synaptic vesicles were isolated, incubated with α-syn, and analyzed using immunoblotting.
Lentivirus production for neuron transduction
This protocol describes the production of lentivirus in HEK293T cells for the transduction of primary neuronal cultures.
Whole-cell patch-clamp recordings
This protocol describes the preparation of hippocampal slices from mice for whole-cell patch-clamp recordings and technique for whole-cell recordings from CA1 pyramidal neurons.
