Output Catalog
ASAP is committed to accelerating the pace of discovery and informing a path to a cure for Parkinson’s disease through collaboration, research-enabling resources, and data sharing. We’ve created this catalog to showcase the research outputs and tools developed by ASAP-funded programs.
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Output Type
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Program
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CRN Team Name
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Theme
Collection of protocols of Team Deleidi used in the publication: “”LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease””
Collection of protocols of Team Deleidi used in the publication: ""LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease""
Available ASAP-related hPSC collection from Team Studer
Collection of human pluripotent stem cell lines consisting of isogenic GBA, LRRK2, SNCA series, KI-reporter lines for TOMM20, b-actin, LAMB1, LAMP1, a-synuclein overexpression lines, and other hPSC resources.
Team Science Approaches to Unravel Monogenic Parkinson’s Disease on a Global Scale
In this article, we describe combining both efforts in a merger project resulting in a global monogenic PD cohort with the buildup of a sustainable infrastructure to identify the multi-ancestry spectrum of monogenic PD and enable studies of factors…
Human Postmortem-Derived Brain Sequencing Collection (Harmonized Collection)
The Human Postmortem-derived Brain Sequencing Collection is a harmonized repository comprised of sequencing data contributed by ASAP CRN teams.
Collection of protocols for paper: “Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function”
This is a collection of protocols used in a recent preprint by the Deleidi Lab, Team Schapira. You can access pre-print at https://doi.org/10.21203/rs.3.rs-1521848/v1
The role of α-synuclein in exocytosis
Review focuses on α-synuclein's role in exocytosis, a key process in Parkinson's disease. Despite unclear pathogenesis, evidence points to αsyn's involvement in regulating cellular processes.
Data for Jain et al Adaptor Protein-3 Produces Synaptic Vesicles that Release Phasic Dopamine
Primary data for Jain et al "Adaptor protein-3 produces synaptic vesicles that release phasic dopamine" (Proc Natl Acad Sci. 2023; 120(42):e2309843120. doi: 10.1073/pnas.2309843120)
Mouse Behavior – Open Field and T-Maze
This protocol describes two behavioral tasks for mice. The first is the Open Field Test, which is used to asses motor behavior, and the second is the T-Maze, which is used to assess spatial learning.
Live Imaging of Primary Mouse Neuron Cultures
This protocol describes live imaging of primary neuron cultures. Included are methods for preparing hippocampal or dopamine neuronal cultures from neonatal mouse brain tissue. The imaging described involves labeling of dopamine neurons with a…
Immunostaining Mouse Brain Tissue or Neuronal Cultures
This protocol describes immunohistochemical and immunoctyochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for…
Mouse Brain Tissue Collection and Analysis
This protocol describes the dissection and collection of coronal sections of the striatum and midbrain from a mouse brain. The tissue can be used in a number of applications and here we describe two: the measurement catecholamine levels using…
Adaptor protein-3 produces synaptic vesicles that release phasic dopamine
The burst firing of midbrain dopamine neurons releases a phasic dopamine signal that mediates reinforcement learning. At many synapses, however, high firing rates deplete synaptic vesicles (SVs), resulting in synaptic depression that limits release.…
Regional Mouse Brain Analysis (Modified QUINT)
This is series of protocols that has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow: QuPath visualization/segmentation QuickNII Brain Atlas Registration QMask Hemispheric Separation Visualign…
BugSigDB – Parkinson’s Disease
A Comprehensive Database of Published Microbial Signatures in PD
Mouse α-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases
Mouse α-synuclein fibrils have been studied to understand Parkinson's disease and multiple system atrophy. They differ from human fibrils in structure and behavior, impacting research on related diseases and potential treatments.
