Albarran et al Histology dataset
By onDataset from a whole-brain imaging experiment showing recombinase expression in different brain regions using knock-in lines (D1-Cre, A2a-Cre, D1-FlpO, A2a-FlpO, DAT-FlpO) crossed with fluorescent reporter lines.
PBMCs isolation with CT Tubes (8mL Whole blood)
By onProtocol isolates peripheral blood mononuclear cells using CPT tubes.
Isolation of Classical Monocytes from PBMCs (MB Kit No. 130-117-337)
By onProtocol isolates classical monocytes from peripheral blood mononuclear cells.
Processing Serum, Plasma, Plasma EDTA from Whole Blood
By onProtocols cover serum, plasma, and plasma EDTA processing from whole blood samples.
Protocol — Quantitative Real-Time PCR (qRT-PCR): 3K-SNCA cells
By onGene expression in 3K-SNCA SH-SY5Y cells was analyzed using Qiagen RNA extraction and TaqMan qRT-PCR on QuantStudio 1 platform for differentiated and undifferentiated cells.
Protocol — LysoTracker® Red DND-99 Live-Cell Staining 26 HCS Analysis
By onText summary: Live-cell lysosomal staining done with LysoTracker Red DND-99, followed by high-content confocal imaging using Opera Phenix Plus, and automated quantification in Harmony/Columbus.
Protocol — DQ™ Red BSA Lysosomal Activity Assay HCS Analysis
By onLysosomal proteolysis can be measured using self-quenched DQ™ Red BSA, followed by confocal imaging for spot-based quantification.
GCase activity assay
By onProtocol measures GCase activity using 4-MUG substrate. GCase protein from a cellular model is diluted in acidic buffer to mimic lysosome pH. Adding lipid/detergent maintains enzyme activity. Taurocholate bile salt is included in the protocol.
Immunofluorescencent Staining of A-synuclein, TFEB, GCase and LAMP1 in treated cultured cells
By onProtocol used to detect a-synuclein, TFEB, GCase, and LAMP1 via immunofluorescence in SH-SY5Y cells with mutant SNCA gene.
Protocols Suite — SH-SY5Y / iCell® Neurons / High-Content Imaging
By onMaintenance of SH-SY5Y cells with SNCA overexpression in DMEM/F-12, differentiation with RA/BDNF, and culture of iCell DopaNeurons with SNCA mutation. Treatment and immunostaining for downstream assays using specific protocols and analysis tools.
Sample Preparation Correlative XPCT – Histology
By onThis protocol describes the placement of samples for XPCT and preparation of correlative histological analysis after the measurement.
Replication Data for: 3D imaging of neuronal inclusions and protein aggregates in human neurodegeneration by multiscale X-ray phase-contrast tomography
By onDataset from DESY, GINX Beamline, Germany offers X-ray phase-contrast tomography volumes of human brain tissue for 3D analysis and reproducible imaging workflow correlation with histological sections.
3d Structure of the Human Hippocampus by holo-tomography: Alzheimer Disease vs Control
By onDataset from ESRF's ID16A beamline includes high-res X-ray nano-tomography data of human brain samples with Alzheimer’s, Parkinson’s, and control cases, along with acquisition details for reuse.
Cryo-EM map of recombinant K58N alpha-synuclein fibrils
By onCryo-EM map obtained from recombinant a-synuclein fibrils carrying the K58N mutation recently discovered in a patient of Parkinson's disease.
Cryo-EM map of in vitro grown WT alpha-synuclein fibrils
By onCryo-EM density map of recombinant WT a-synuclein fibrils at 2.7 A resolution.
Cryo-EM map of in vitro grown G14R alpha-synuclein fibrils
By on3.0 A resolution cryo-EM map obtained from recombinant a-synuclein fibrils carrying the G14R mutation recently discovered in a patient of a complex neurological disease.
Tomogram of aggregate in AgDD-sfGFP-expressing HEK293 cell 6 h post aggregation induction
By onAgDD protein aggregation in HEK293 cells can be temporally controlled for studying cellular effects. A cryo-electron tomogram shows AgDD aggregate formation 6h post-induction.
Tomogram of aggregate in AgDD-sfGFP-expressing HEK293 cell 10 min post aggregation induction
By onAgDD protein aggregation can be controlled for cellular studies. A cryo-electron tomogram shows an AgDD aggregate in a HEK293 cell 10 min post-aggregation induction.
